The objective of this project is to explore the feasibility of polarization signal imaging of early receptor potential (ERP) in intact retinas. Previous investigations have demonstrated the existence of polarization signal response due to ERP-associated birefringence change in isolated photoreceptor outer segments activated by visible light. However, imaging of the ERP-associated birefringence change in intact retina is technically challenging because of complex structure of the retina. In principle, both birefringence and scattering changes can produce transient polarization signals in the retina. In order to achieve robust imaging of ERP-associated birefringence change, it is important to reduce the effect of light scattering on the measurement. In this project, we propose to develop a high speed light-field optical coherence tomography (OCT) imager, and to validate it for optical separation of birefringence- and scattering-associated polarization signals. The proposed light-field OCT imager will provide capability of angle-resolved polarization signal imaging. In principle, when an oblique light illumination is used, synthetic angular filters will allow optical separation of light scattering- and birefringence-associated polarization signals in the light activated retina. Isolated lobster nerve axons will be employed as a transition preparation for functional test of the proposed imager. Frog retinal slices and intact retinas will be used to demonstrate the feasibility of polarization signal imaging of ERP-associated birefringence response. Although our ultimate goal is to use the proposed technology to diagnose of retinal diseases, this R21 project will focus on technical development and functional validation of the light-field imaging system. Successful implementation of this project will build a solid foundation for the development of objective, high resolution instruments for photoreceptor function evaluation.

Public Health Relevance

The proposed light-field imager will provide capability of high resolution imaging of stimulus-evoked early receptor potential. High resolution evaluation of photoreceptor response can lead to better study and diagnosis of the retina.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21EB012264-01A1
Application #
8112263
Study Section
Special Emphasis Panel (ZRG1-SBIB-J (80))
Program Officer
Conroy, Richard
Project Start
2011-04-01
Project End
2013-03-31
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
1
Fiscal Year
2011
Total Cost
$198,360
Indirect Cost
Name
University of Alabama Birmingham
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
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Lu, Rongwen; Levy, Alexander M; Zhang, Qiuxiang et al. (2013) Dynamic near-infrared imaging reveals transient phototropic change in retinal rod photoreceptors. J Biomed Opt 18:106013
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Lu, Rong-Wen; Curcio, Christine A; Zhang, Youwen et al. (2012) Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina. J Biomed Opt 17:060504
Yao, Xin-Cheng; Li, Yi-Chao (2012) Functional imaging of retinal photoreceptors and inner neurons using stimulus-evoked intrinsic optical signals. Methods Mol Biol 884:277-85
Yao, Xin-Cheng; Cui, Wan-Xing; Li, Yi-Chao et al. (2012) Functional imaging of glucose-evoked rat islet activities using transient intrinsic optical signals. J Mod Opt 59:

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