There is a growing need for a highly efficient, simple, and easy to use quantitation method for multiplexed marker analyses. We hypothesize that differences in analyte flux to different addresses in a heterogeneous array-based platform can serve as an effective means to simultaneously determine the absolute concentration of a number of analytes with only the need to add a single calibrant to the sample. This proposal details a reduction to practice of technology that will afford a simple and cost-effective approach to determine the concentration of each marker - irrespective of the number of markers - without requiring the creation of calibration curves or the use of calibrants. The platform is extensible - meaning it could be deployed for genomic and metabolic studies as well as for platform proteomic assays - and could be applied to other readout methods.

Public Health Relevance

Breakthroughs in methodologies for calibration will prove pivotal as health care management begins to more strongly rely on multiplexed tests, placing a premium on sample consumption and throughput, labor, and material and reagent costs. This proposal details a reduction to practice of technology that will afford a simple and cost-effective approach to determine the concentration of each marker - irrespective of the number of markers - without requiring the creation of calibration curves or the use of calibrants.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21EB016925-01
Application #
8493750
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Korte, Brenda
Project Start
2013-04-01
Project End
2015-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
1
Fiscal Year
2013
Total Cost
$223,688
Indirect Cost
$73,688
Name
University of Utah
Department
Internal Medicine/Medicine
Type
Organized Research Units
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112