The goal of the proposed project is to understand the protein composition of ribonucleoprotein complexes containing microRNA miR-29b. MicroRNAs are endogenous, small regulatory RNA molecules. Numerous recent studies have demonstrated that microRNAs are important regulators of gene expression and play crucial roles in a wide range of physiological processes and diseases including cardiovascular and renal diseases. The effect of microRNAs on target genes is mediated by interactions of microRNAs with proteins that, together, form microRNA-containing ribonucleoprotein complexes (miRNPs). Despite the crucial and wide-spread functional role of microRNAs, the number of protein components of miRNPs that have been characterized is surprisingly small. The understanding of proteins interacting with specific microRNAs is particularly lacking, despite the recognition that different microRNAs may act differently. The shortage of knowledge in this critical area significantly hampers in-depth understanding of how microRNAs act, how the action of microRNAs is regulated, and how microRNAs contribute to the regulation of physiology and disease. We propose to develop a new approach for identifying protein components of miRNPs specifically containing miR-29b, a microRNA that we have shown to be functionally important in the protection against hypertensive renal injury.
Aim 1 will develop the approach, identify the protein components, and provide initial characterization of the newly identified proteins.
Aim 2 will examine the functional significance of the newly identified proteins.

Public Health Relevance

The result of the proposed study could significantly improve our understanding of how the action of microRNAs is mediated and enable in-depth studies of how microRNAs contribute to physiological regulation and the development of disease.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Exploratory/Developmental Grants (R21)
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Pathobiology of Kidney Disease Study Section (PBKD)
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OH, Youngsuk
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Medical College of Wisconsin
Schools of Medicine
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Liu, Yong; Usa, Kristie; Wang, Feng et al. (2018) MicroRNA-214-3p in the Kidney Contributes to the Development of Hypertension. J Am Soc Nephrol 29:2518-2528
Kriegel, Alison J; Terhune, Scott S; Greene, Andrew S et al. (2018) Isomer-specific effect of microRNA miR-29b on nuclear morphology. J Biol Chem 293:14080-14088
Liu, Yong; Liu, Pengyuan; Yang, Chun et al. (2014) Base-resolution maps of 5-methylcytosine and 5-hydroxymethylcytosine in Dahl S rats: effect of salt and genomic sequence. Hypertension 63:827-38
Wang, Feng; Li, Liping; Xu, Haiming et al. (2014) Characteristics of long non-coding RNAs in the Brown Norway rat and alterations in the Dahl salt-sensitive rat. Sci Rep 4:7146
Mladinov, Domagoj; Liu, Yong; Mattson, David L et al. (2013) MicroRNAs contribute to the maintenance of cell-type-specific physiological characteristics: miR-192 targets Na+/K+-ATPase ?1. Nucleic Acids Res 41:1273-83
Kriegel, Alison J; Liu, Yong; Liu, Pengyuan et al. (2013) Characteristics of microRNAs enriched in specific cell types and primary tissue types in solid organs. Physiol Genomics 45:1144-56
Kriegel, Alison J; Liang, Mingyu (2013) MicroRNA in situ hybridization for formalin fixed kidney tissues. J Vis Exp :
Liang, Mingyu; Cowley Jr, Allen W; Mattson, David L et al. (2013) Epigenomics of hypertension. Semin Nephrol 33:392-9
Jia, Ping; Teng, Jie; Zou, Jianzhou et al. (2013) miR-21 contributes to xenon-conferred amelioration of renal ischemiaýýýreperfusion injury in mice. Anesthesiology 119:621-30
Jia, Ping; Teng, Jie; Zou, Jianzhou et al. (2013) Intermittent exposure to xenon protects against gentamicin-induced nephrotoxicity. PLoS One 8:e64329

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