Surgical resection of glioblastomas (GBM) fails to completely remove invasive tumor tissue, resulting in tumor recurrence. Virus vector delivery of anti-tumor genes to remaining malignant cells has potential to prevent this recurrence. An enduring issue with virus vectors is inefficient transgene delivery throughout the tumor after direct injection. As the brain and brain tumors are both highly vascularized, delivery of therapies via the vasculature is an attractive alternative approach for widespread transgene delivery. However, current vector systems are suboptimal for intravascular (i.v.) gene delivery to the brain due to liver uptake, the impenetrability of the blood-brain barrier (BBB), and pre-exisiting immunity to the virus (e.g. neutralizing antibodies to the virus capsid). We therefore sought alternative strategies which would serve two purposes: (1) to allow specific targeting of adeno-associated virus (AAV) vector to the brain tumor environment (BTE) and (2) shield AAV capsids from neutralizing antibodies. Microvesicles (MV) are naturally secreted, membrane-encompassed structures known to deliver proteins, RNA, miRNA, and DNA to neighboring cells. Accumulating research suggests they may be suitable for targeted gene/protein transfer. We have discovered that during vector production, a portion of AAV isolated from the media is associated with MV. AAV capsids were localized primarily on the inside of MV by transmission electron microscopic examination. We have found that the MV-associated AAV, termed MV-AAV, are more efficient at gene delivery to cultured cells compared to standard cell lysate purified AAV vectors. We have also shown that surface tagging MV-AAV with magnetic nanoparticles allows for directional AAV-mediated gene transfer via the use of a magnet placed on the bottom of the cell culture well. Furthermore a recent study showed that MV's loaded with siRNA could be specifically targeted to the brain after i.v. injection in mice. This proposal will test the hypothesis that MV-AAV can be targeted to the brain tumor environment after i.v. injection to therapeutically treat the tumor. This work has potential to provide a non-invasive means of robust and global gene delivery to the brain.

Public Health Relevance

In this proposal we will engineer targeted microvesicle-associated AAV (MV-AAV) gene delivery vehicles for gene therapy against glioblastoma multiforme (GBM) brain tumors in mice. By expressing ligands known to cross the blood-brain barrier (BBB) on the MV surface we anticipate the AAV vector to preferentially deliver genes to the brain tumor environment. We will initially analyze brain targeting efficiency using bioluminescence imaging of AAV vector-encoded firefly luciferase expression. Next using the most efficient targeting approach, we will perform anti-GBM gene therapy by using a combined drug/AAV encoded anti-tumor protein therapy. This study has broad implications for cancer and neurodegenerative diseases where global gene therapy to the brain is desired.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21NS081374-02
Application #
8536422
Study Section
Molecular Neurogenetics Study Section (MNG)
Program Officer
Fountain, Jane W
Project Start
2012-09-01
Project End
2014-08-30
Budget Start
2013-08-31
Budget End
2014-08-30
Support Year
2
Fiscal Year
2013
Total Cost
$209,888
Indirect Cost
$89,263
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Maguire, Casey A; Ramirez, Servio H; Merkel, Steven F et al. (2014) Gene therapy for the nervous system: challenges and new strategies. Neurotherapeutics 11:817-39
Maguire, Casey A (2014) Bioluminescence-based monitoring of virus vector-mediated gene transfer in mice. Methods Mol Biol 1098:197-209
Gy├Ârgy, Bence; Fitzpatrick, Zachary; Crommentuijn, Matheus H W et al. (2014) Naturally enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery in vivo. Biomaterials 35:7598-609
Maguire, Casey A; Crommentuijn, Matheus Hw; Mu, Dakai et al. (2013) Mouse gender influences brain transduction by intravascularly administered AAV9. Mol Ther 21:1470-1
Atai, Nadia A; Balaj, Leonora; van Veen, Henk et al. (2013) Heparin blocks transfer of extracellular vesicles between donor and recipient cells. J Neurooncol 115:343-51