The University of Illinois at Chicago has unique facilities and expertise for specialized liquid chromatography-tandem mass spectrometry (LC-MS-MS) measurements in support of cancer research including a unique LC-MS-MS assay for DNA oxidation products as surrogate end-point biomarkers for cancer prevention, and mass spectrometric-based screening of natural product mixtures and metabolite mixtures for the discovery of cancer chemoprevention agents. Existing mass spectrometry instrumentation for this resource includes two LC-MS quadrupole instruments, one magnetic sector mass spectrometer and a new electrospray triple quadrupole mass spectrometer. The instruments are managed by the P.I. who is both a tenured mass spectrometrist in the College of Pharmacy and an NCI-funded cancer researcher. Because no other mass spectrometry laboratory in the Chicago area is directed by a biomedical mass spectrometrist nor a cancer researcher, unique opportunities exist for providing analytical and educational support for cancer research. Developed in the laboratory of the P.I., the LC-MS-MS assay for DNA oxidation products simultaneously measures four oxidized nucleosides including 8-oxo-2'-deoxyguanosine (the only oxidation product measured by the standard HPLC with electrochemical detection method), 8-oxo-2'-deoxyadenosine, thymidine glycol and 5-hydroxymethyl-2'-uridine. Invented in the laboratory of the P.I., pulsed ultrafiltration mass spectrometry facilitates high throughput screening of natural product extracts as a means of drug discovery. Funds are requested to make these resources available to other cancer researchers on campus. In particular, the research of seven NCI-funded investigators is described with reference to how access to this unique mass spectrometry resource will increase their productivity. These research activities encompass both preclinical and clinical studies and include screening of natural products for potential new cancer chemoprevention agents, cancer prevention by selenium, mechanisms of carcinogenesis by estrogens, tamoxifen chemoprevention of breast cancer, and clinical investigations of cancer chemoprevention by the carotenoid lycopene.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Resource-Related Research Projects (R24)
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Special Emphasis Panel (ZCA1-SRRB-C (M1))
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Seifried, Harold E
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University of Illinois at Chicago
Schools of Pharmacy
United States
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van Breemen, Richard B; Sharifi, Roohollah; Viana, Marlos et al. (2011) Antioxidant effects of lycopene in African American men with prostate cancer or benign prostate hyperplasia: a randomized, controlled trial. Cancer Prev Res (Phila) 4:711-8
Sun, Yongkai; Gu, Chungang; Liu, Xuemei et al. (2005) Ultrafiltration tandem mass spectrometry of estrogens for characterization of structure and affinity for human estrogen receptors. J Am Soc Mass Spectrom 16:271-9
Li, Yan; Yang, Xiaofeng; Chang, Minsun et al. (2005) Functional and structural comparisons of cysteine residues in the Val108 wild type and Met108 variant of human soluble catechol O-methyltransferase. Chem Biol Interact 152:151-63
Li, Yan; Yang, Xiaofeng; van Breemen, Richard B et al. (2005) Characterization of two new variants of human catechol O-methyltransferase in vitro. Cancer Lett 230:81-9
Zhu, Yaoqiu; Nikolic, Dejan; Van Breemen, Richard B et al. (2005) Mechanism of inactivation of inducible nitric oxide synthase by amidines. Irreversible enzyme inactivation without inactivator modification. J Am Chem Soc 127:858-68
Li, Yan; Yao, Jiaqin; Chang, Minsun et al. (2004) Equine catechol estrogen 4-hydroxyequilenin is a more potent inhibitor of the variant form of catechol-O-methyltransferase. Chem Res Toxicol 17:512-20
Yu, Linning; Liu, Hong; Li, Wenkui et al. (2004) Oxidation of raloxifene to quinoids: potential toxic pathways via a diquinone methide and o-quinones. Chem Res Toxicol 17:879-88
Li, Y; Grubjesic, S; Nikolic, D et al. (2004) In vitro assessment of intestinal permeability and hepatic metabolism of 4'-bromoflavone, a promising cancer chemopreventive agent. Xenobiotica 34:535-47
Liu, Xuemei; Zhang, Fagen; Liu, Hong et al. (2003) Effect of halogenated substituents on the metabolism and estrogenic effects of the equine estrogen, equilenin. Chem Res Toxicol 16:741-9
Yu, Chongwoo; Shin, Young Geun; Kosmeder, Jerome W et al. (2003) Liquid chromatography/tandem mass spectrometric determination of inhibition of human cytochrome P450 isozymes by resveratrol and resveratrol-3-sulfate. Rapid Commun Mass Spectrom 17:307-13

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