Due to the complexity of type 1 diabetes mellitus (T1D), successful treatment will require a paradigm shifting multidisciplinary approach. The discovery of novel targets, the development of efficient drug delivery systems and the integration of state-of-the-art imaging techniques will provide real-time monitoring of both drug delivery and therapeutic outcome. Conventional therapeutics are ineffective due, in part, to the lack of directed targeting to the islets, resulting in low bioavailability. This inefficient delivey necessitates systemic administration of high doses of therapy and consequently untoward off-target effects and toxicity. To overcome these severe limitations, we propose to develop efficient delivery systems with imaging capabilities that specifically direct siRNA-based therapies to insulin-producing beta-cells. Our strategy for directed therapy is based on the exquisite specificity of RNAi for silencing harmful disease-promoting genes and our extensive experience in developing image-guided systems for siRNA delivery in various settings. Here we propose to develop an image-assisted approach for delivering siRNA therapy to endogenous beta- cells using a targeted magnetic nanoparticle platform (Nanodrug, ND) based on dextran coated iron oxide nanoparticles. These will serve as vehicles for specific delivery of siRNA to pancreatic beta-cells by targeting recently identified markers on beta-cells (ND-siRNA). In addition, these nanodrugs will function as imaging reporters for in vivo monitoring of delivery by optical and magnetic resonance imaging (MRI). We will first generate luciferase (luc)-conjugated beta-cell-specific moieties that target beta-cells and utilize bioluminescence imaging (BLI) to test their in vivo ability to label beta-cells. Next, we will attach selected targeting moieties to siRNA-containing magnetic nanoparticles and test their ability to deliver siRNA to isolated islets in culture and then in animal models of T1D concurrently with in vivo monitoring of delivery. Genes that are responsible for beta-cell apoptosis and immune recognition will be targeted with siRNA. We expect to observe a decreased T1D incidence in prevention studies and increased number of animals with restored normoglycemia in reversal studies.

Public Health Relevance

Due to the complexity of type 1 diabetes mellitus, successful treatment will require a multidisciplinary approach that combines the discovery of novel targets, and the development of efficient drug delivery systems in combination with state-of-the-art imaging techniques. The goal of this study is to develop novel efficient image-guided delivery systems to direct siRNA therapies specifically to insulin-producing pancreatic beta-cells. In addition, we propose to monitor the delivery with non-invasive imaging, which will allow for precise localization of the therapeutics at the target site. This will be done by a collaborative team of investigators from Massachusetts General Hospital, Stanford University Medical School and University of [Massachusetts Medical School.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects (R24)
Project #
5R24DK096465-02
Application #
8703684
Study Section
Special Emphasis Panel (ZDK1)
Program Officer
Laughlin, Maren R
Project Start
2013-07-18
Project End
2015-06-30
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Bronsart, Laura L; Stokes, Christian; Contag, Christopher H (2016) Multimodality Imaging of Cancer Superoxide Anion Using the Small Molecule Coelenterazine. Mol Imaging Biol 18:166-71
Bronsart, Laura L; Stokes, Christian; Contag, Christopher H (2016) Chemiluminescence Imaging of Superoxide Anion Detects Beta-Cell Function and Mass. PLoS One 11:e0146601
Bronsart, Laura L; Contag, Christopher H (2016) A role of the adaptive immune system in glucose homeostasis. BMJ Open Diabetes Res Care 4:e000136
Wang, Ping; Yoo, Byunghee; Yang, Jingsheng et al. (2014) GLP-1R-targeting magnetic nanoparticles for pancreatic islet imaging. Diabetes 63:1465-74