Abstract

Dr. Zhang proposes to compare the rate of mutation in vivo during first and second strand DNA synthesis. He will utilize an in vitro assay to compare the frameshift rates of RNA polymerase II, RT first strand, and RT second strand synthesis. He will test the three prevailing models for how recombination occurs in retroviruses using in vivo and in vitro systems. He will also test the effects of RNA secondary structure on stimulation of recombination.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA070407-03
Application #
2895526
Study Section
Experimental Virology Study Section (EVR)
Program Officer
Cole, John S
Project Start
1997-08-01
Project End
2002-07-31
Budget Start
1999-08-01
Budget End
2000-07-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Kentucky
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Zhang, Jiayou (2004) Host RNA polymerase II makes minimal contributions to retroviral frame-shift mutations. J Gen Virol 85:2389-95
Li, Ting; Zhang, Jiayou (2004) Stable expression of three genes from a tricistronic retroviral vector containing a picornavirus and 9-nt cellular internal ribosome entry site elements. J Virol Methods 115:137-44
Li, Ting; Zhang, Jiayou (2002) Intramolecular recombinations of Moloney murine leukemia virus occur during minus-strand DNA synthesis. J Virol 76:9614-23
Li, T; Zhang, J (2001) Retroviral recombination is temperature dependent. J Gen Virol 82:1359-64
Zhang, J; Ma, Y (2001) Evidence for retroviral intramolecular recombinations. J Virol 75:6348-58
Zhang, J; Sapp, C M (2001) A novel retroviral vector that allows the magnetic selection of infected cells. J Virol Methods 94:1-6
Tang, L Y; Zhang, J (2000) The cellular mismatch repair system is able to repair mismatches within MLV retroviral double-stranded DNA at a low frequency. Nucleic Acids Res 28:2302-6
Zhang, J; Tang, L Y; Li, T et al. (2000) Most retroviral recombinations occur during minus-strand DNA synthesis. J Virol 74:2313-22
Zhang, J; Sapp, C M (1999) Recombination between two identical sequences within the same retroviral RNA molecule. J Virol 73:5912-7
Sapp, C M; Li, T; Zhang, J (1999) Systematic comparison of a color reporter gene and drug resistance genes for the determination of retroviral titers. J Biomed Sci 6:342-8

Showing the most recent 10 out of 12 publications