Abstract

This application outlines a series of experiments The applicant intends to outline hypotheses for the cell biological functions of myosin-X by determining its localization in tissues and cells. He will then define the basic structural and biochemical properties of this class of myosin and its tail domain and test whether purified myosin-X actually functions as a molecular motor in in vitro motility assays.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
1R29DC003299-01
Application #
2014864
Study Section
Biological Sciences 2 (BIOL)
Project Start
1997-06-01
Project End
2002-05-31
Budget Start
1997-06-01
Budget End
1998-05-31
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Physiology
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Bultema, Jarred J; Boyle, Judith A; Malenke, Parker B et al. (2014) Myosin vc interacts with Rab32 and Rab38 proteins and works in the biogenesis and secretion of melanosomes. J Biol Chem 289:33513-28
Lin, Wan-Hsin; Hurley, Joshua T; Raines, Alexander N et al. (2013) Myosin X and its motorless isoform differentially modulate dendritic spine development by regulating trafficking and retention of vasodilator-stimulated phosphoprotein. J Cell Sci 126:4756-68
Liu, Katy C; Jacobs, Damon T; Dunn, Brian D et al. (2012) Myosin-X functions in polarized epithelial cells. Mol Biol Cell 23:1675-87
Quintero, Omar A; DiVito, Melinda M; Adikes, Rebecca C et al. (2009) Human Myo19 is a novel myosin that associates with mitochondria. Curr Biol 19:2008-13
Xie, Jiansong; Marchelletta, Ronald R; Thomas, Padmaja B et al. (2009) Transduced viral IL-10 is exocytosed from lacrimal acinar secretory vesicles in a myosin-dependent manner in response to carbachol. Exp Eye Res 88:467-78
Jacobs, Damon T; Weigert, Roberto; Grode, Kyle D et al. (2009) Myosin Vc is a molecular motor that functions in secretory granule trafficking. Mol Biol Cell 20:4471-88