Plants and animals respond to MAMPs (microbe-associated molecular patterns). MAMP-activated response pathways are functionally analogous to mammalian TLR-signaling. A high throughput Arabidopsis seedling assay allowed us to make several important discoveries. First, bacterial flagellin (Flg22) elicits the deposition of callose in cotyledons via a pathway that involves the transcription factor MYB51, ethylene signaling, and the synthesis of indole glucosinolates that function as novel secondary messengers. Second, bacterial elongation factor EF-Tu (Efl26) and Flg22 elicit callose production by distinct pathways. Third, Flg22, but not oligogalacturonides (OGs), elicit the production of soluble anti-microbial compound(s). Fourth, the bacterial phytopathogen Pseudomonas syringae blocks MAMP signaling in roots by the production of the jasmonic acid (JA) mimic coronatine. In addition, we have also shown:1) that cell-wall associated peroxidases play a key role in the plant defense response, 2) that infection of Arabidopsis by the opportunistic broad host range pathogen Pseudomonas aerugionsa involves novel cell wall degrading enzymes, and 3) that the insect leaf miner Scaptomyza graminum, which is nested in the Drosophila phylogenetic clade, is an aggressive Arabidopsis herbivore. In the second round of MERIT funding: We will investigate how MAMP-activated pathways relate to defense pathways mediated by salicylic acid, JA and ET. We will determine the molecular mechanism by which coronatine blocks MAMP signaling. Using MYB51 as a model, we will identify the set of proteins required to activate MYB51-responsive promoters. We will identify the soluble antimicrobial factors elicited by MAMPs and elucidate the signaling pathway(s) leading to their synthesis. We will distinguish the roles that peroxidases and NADPH oxidases play in plant defense and use the Scaptomyza model to determine the role of the oxidase burst in insect defense. We will identify P. aeruginosa cell wall degrading enzymes and determine the role(s) they play in plant pathogenesis.

Public Health Relevance

Although adaptive immunity is unique to vertebrates, the innate immune response has ancient origins. Common features of innate immunity in plants and animals include defined receptors for microbe-associated molecules, conserved MAPK signaling cascades, and the production of anti-microbial peptides and compounds. Studying innate immunity in non-vertebrates enhances our understanding of human immunity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM048707-22
Application #
8600685
Study Section
Special Emphasis Panel (NSS)
Program Officer
Sledjeski, Darren D
Project Start
1993-01-01
Project End
2014-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
22
Fiscal Year
2014
Total Cost
$538,872
Indirect Cost
$234,424
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Groen, Simon C; Humphrey, Parris T; Chevasco, Daniela et al. (2016) Pseudomonas syringae enhances herbivory by suppressing the reactive oxygen burst in Arabidopsis. J Insect Physiol 84:90-102
Liu, Simu; Bartnikas, Lisa M; Volko, Sigrid M et al. (2016) Mutation of the Glucosinolate Biosynthesis Enzyme Cytochrome P450 83A1 Monooxygenase Increases Camalexin Accumulation and Powdery Mildew Resistance. Front Plant Sci 7:227
Haney, Cara H; Samuel, Buck S; Bush, Jenifer et al. (2015) Associations with rhizosphere bacteria can confer an adaptive advantage to plants. Nat Plants 1:
Benedetti, Manuel; Pontiggia, Daniela; Raggi, Sara et al. (2015) Plant immunity triggered by engineered in vivo release of oligogalacturonides, damage-associated molecular patterns. Proc Natl Acad Sci U S A 112:5533-8
Rellán-Álvarez, Rubén; Lobet, Guillaume; Lindner, Heike et al. (2015) GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown root systems. Elife 4:
Cheng, Zhenyu; Li, Jian-Feng; Niu, Yajie et al. (2015) Pathogen-secreted proteases activate a novel plant immune pathway. Nature 521:213-6
Rajniak, Jakub; Barco, Brenden; Clay, Nicole K et al. (2015) A new cyanogenic metabolite in Arabidopsis required for inducible pathogen defence. Nature 525:376-9
Zhang, Xue-Cheng; Millet, Yves A; Cheng, Zhenyu et al. (2015) Jasmonate signalling in Arabidopsis involves SGT1b-HSP70-HSP90 chaperone complexes. Nat Plants 1:
Mammarella, Nicole D; Cheng, Zhenyu; Fu, Zheng Qing et al. (2015) Apoplastic peroxidases are required for salicylic acid-mediated defense against Pseudomonas syringae. Phytochemistry 112:110-21
Cole, Stephanie J; Yoon, Alexander J; Faull, Kym F et al. (2014) Host perception of jasmonates promotes infection by Fusarium oxysporum formae speciales that produce isoleucine- and leucine-conjugated jasmonates. Mol Plant Pathol 15:589-600

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