Renln-expresslng cells are crucial in the control of blood pressure, fluid and electrolyte homeostasis and development ofthe kidney vasculature. Understanding the molecular mechanisms governing the acquisition of renin cell identity-and their plasticity to switch the renin phenotype on and off- is of fundamental biological and medical relevance. With the support of the R37 we generated mouse and cell models which allowed us to demonstrate that the identity and plasticity ofthe renin cell is determined by chromatin remodeling at the renin gene resulting from the balance between histone methylation and acetylation marks at the renin promoter. We further showed that cAMP plays a central role in the regulation of renin expression and renin cell identity and that the transcriptional effects of cAMP are mediated by the binding of CREB and histone acetyl transferases CBP/p300 to the cAMP response element (CRE) located in the enhancer region ofthe renin gene. While there is still work to be completed, the results obtained during the current cycle allow us to formulate with confidence a plan that will expand the depth of our understanding as to how renin cell identity is regulated. The overall hypothesis to be tested is that the identity of the renin cell is determined by the three- dimensional conformation of chromatin governed by: 1) renin gene locus specific and associated genome- wide epigenetic marks such as histone acetylation and methylation and DNA methylation patterns, 2) the establishment of gene-gene interactions and transcriptional factories ultimately responsible for the coordinated expression ofthe genes that confer the renin cell with its unique identity, and 3) interactions of the CRE (and associated Creb/CBP/p300) of the renin gene enhancer with regulatory elements within the renin gene (RBP-J, Hox/Pbx sites) and other genes in the genome are ultimately responsible for the pattern of unique genome-wide epigenetic changes, chromosomal looping and transcriptional factories that determine the identity and fate of the renin cell.
The proposed work should provide fundamental new information regarding the molecular-genomic mechanisms that control renin cell identity and plasticity. Novel information gained form these studies may be of benefit to individuals affected by hypertension, cardiovascular diseases involving tissue repair, vascular development and renal diseases resulting from abnormal vascular structure and/or differentiation.
|Gomez, R Ariel; Belyea, Brian; Medrano, Silvia et al. (2014) Fate and plasticity of renin precursors in development and disease. Pediatr Nephrol 29:721-6|
|Belyea, Brian C; Xu, Fang; Pentz, Ellen S et al. (2014) Identification of renin progenitors in the mouse bone marrow that give rise to B-cell leukaemia. Nat Commun 5:3273|
|Lin, E E; Sequeira-Lopez, M L S; Gomez, R A (2014) RBP-J in FOXD1+ renal stromal progenitors is crucial for the proper development and assembly of the kidney vasculature and glomerular mesangial cells. Am J Physiol Renal Physiol 306:F249-58|
|Moniwa, Norihito; Varagic, Jasmina; Ahmad, Sarfaraz et al. (2013) Hemodynamic and hormonal changes to dual renin-angiotensin system inhibition in experimental hypertension. Hypertension 61:417-24|
|Kurt, Birguel; Paliege, Alexander; Willam, Carsten et al. (2013) Deletion of von Hippel-Lindau protein converts renin-producing cells into erythropoietin-producing cells. J Am Soc Nephrol 24:433-44|
|Pentz, Ellen Steward; Cordaillat, Magali; Carretero, Oscar A et al. (2012) Histone acetyl transferases CBP and p300 are necessary for maintenance of renin cell identity and transformation of smooth muscle cells to the renin phenotype. Am J Physiol Heart Circ Physiol 302:H2545-52|
|Medrano, Silvia; Monteagudo, Maria C; Sequeira-Lopez, Maria Luisa S et al. (2012) Two microRNAs, miR-330 and miR-125b-5p, mark the juxtaglomerular cell and balance its smooth muscle phenotype. Am J Physiol Renal Physiol 302:F29-37|
|Kurt, Birguel; Kurtz, Lisa; Sequeira-Lopez, Maria L et al. (2011) Reciprocal expression of connexin 40 and 45 during phenotypical changes in renin-secreting cells. Am J Physiol Renal Physiol 300:F743-8|
|Xu, Di; Borges, Giulianna R; Davis, Deborah R et al. (2011) Neuron- or glial-specific ablation of secreted renin does not affect renal renin, baseline arterial pressure, or metabolism. Physiol Genomics 43:286-94|
|Castellanos Rivera, Ruth M; Monteagudo, Maria C; Pentz, Ellen S et al. (2011) Transcriptional regulator RBP-J regulates the number and plasticity of renin cells. Physiol Genomics 43:1021-8|
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