One of the most challenging, yet highly rewarding tasks in biological research is to visualize with high spatial resolution the structure of molecula machines and their interactions in cellular function under physiological conditions, such as in 37 ?C aqueous environment. This proposed LiquidCell EM system with precise temperature control enables direct imaging of many dynamic biological processes in truly native environments with ultra high resolution using transmission electron microscopy. In this proposal, we will fabricate the self-contained liquid cells with ultra-thin viewing windows. Precise temperature control of 37+/-0.1 ?C will be achieved through microfluidic device fabrication. Test experiments of imaging individual proteins, protein assembly and live cell functions will be performed to optimize the system for in vivo biological imaging under physiological conditions.

Agency
National Institute of Health (NIH)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43GM109752-01A1
Application #
8779700
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Wu, Mary Ann
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Zinstruments, LLC
Department
Type
DUNS #
City
Berkeley
State
CA
Country
United States
Zip Code
94708