We request funds for a new inductively coupled plasma mass spectrometer (ICP-MS) interfaced to a high- performance liquid chromatography (HPLC) system to be located at the Athinoula A. Martinos Center for Biomedical Imaging at Massachusetts General Hospital (MGH). ICP-MS is a powerful analytical tool for the detection and quantification of trace elements in a broad range of sample types and is widely accepted as the method of choice for elemental analysis. Modern instrumentation can analyze up to 60 elements simultaneously with a dynamic range spanning 9 orders of magnitude from parts per trillion to parts per thousand. An HPLC interface enables speciation of metal or metalloid analytes in complex matrices like blood plasma or tissue homogenate. The dual mission of the Martinos Center is technology development and translational research involving advanced imaging techniques such as magnetic resonance imaging (MRI), positron emission tomography, optical imaging, and magnetoencephalography. Accurate elemental analysis underpins much of this work. For instance in molecular imaging probe development, quantitative elemental analysis is used to characterize the probes, quantify their distribution, pharmacokinetics, and metabolism in tissue. For imaging of metal ion homeostasis, ICP-MS provides a crucial gold standard. Development of metal-based therapeutics benefits tremendously from HPLC-ICP-MS for metabolism studies. Contrast agents are widely used in the development of quantitative in vivo imaging techniques, e.g. perfusion, permeability, blood volume measurements;ex vivo elemental analysis of these contrast agents can serve to calibrate and validate these methodologies. The requested instrumentation will be administered as a shared resource for the Martinos center, the larger MGH research community, as well as other institutions in the greater Boston area. The Principle Investigator and Advisory Committee will ensure both equitable and cost-effective operation of this system. The acquisition of this HPLC-ICP-MS will greatly benefit our research community and we expect it will play an essential role in driving many new discoveries pertaining to a host of medically related issues including cancer, diabetes, drug addiction, Alzheimer's disease, heart disease, liver disease, stroke, and neural plasticity. PUBLIC HEALTH REELEVANCE: Funds are requested for an elemental analyzer that will allow quantification and distribution of metals and metalloids in biological tissue. The analyses enabled by this instrument will underpin NIH-funded research projects in cancer, heart disease, diabetes, addiction research, Alzheimer's disease, and neuroplasticity.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD010650-01A1
Application #
8447659
Study Section
Special Emphasis Panel (ZRG1-BCMB-D (30))
Program Officer
Birken, Steven
Project Start
2013-06-15
Project End
2014-06-14
Budget Start
2013-06-15
Budget End
2014-06-14
Support Year
1
Fiscal Year
2013
Total Cost
$371,180
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Waghorn, Philip A; Jones, Chloe M; Rotile, Nicholas J et al. (2017) Molecular Magnetic Resonance Imaging of Lung Fibrogenesis with an Oxyamine-Based Probe. Angew Chem Int Ed Engl 56:9825-9828
Shea, Barry S; Probst, Clemens K; Brazee, Patricia L et al. (2017) Uncoupling of the profibrotic and hemostatic effects of thrombin in lung fibrosis. JCI Insight 2:
Boros, Eszter; Srinivas, Raja; Kim, Hee-Kyung et al. (2017) Intramolecular Hydrogen Bonding Restricts Gd-Aqua-Ligand Dynamics. Angew Chem Int Ed Engl 56:5603-5606
Adams, Casey J; Wilson, Justin J; Boros, Eszter (2017) Multifunctional Desferrichrome Analogues as Versatile 89Zr(IV) Chelators for ImmunoPET Probe Development. Mol Pharm 14:2831-2842
Oliveira, Bruno L; Caravan, Peter (2017) Peptide-based fibrin-targeting probes for thrombus imaging. Dalton Trans 46:14488-14508
Shen, Shiqian; Lim, Grewo; You, Zerong et al. (2017) Gut microbiota is critical for the induction of chemotherapy-induced pain. Nat Neurosci 20:1213-1216
Désogère, Pauline; Tapias, Luis F; Hariri, Lida P et al. (2017) Type I collagen-targeted PET probe for pulmonary fibrosis detection and staging in preclinical models. Sci Transl Med 9:
Dos Santos Ferreira, Diego; Jesus de Oliveira Pinto, Bruno Luís; Kumar, Vidhya et al. (2017) Evaluation of antitumor activity and cardiac toxicity of a bone-targeted ph-sensitive liposomal formulation in a bone metastasis tumor model in mice. Nanomedicine 13:1693-1701
Waghorn, Philip A; Oliveira, Bruno L; Jones, Chloe M et al. (2017) High sensitivity HPLC method for determination of the allysine concentration in tissue by use of a naphthol derivative. J Chromatogr B Analyt Technol Biomed Life Sci 1064:7-13
Gale, Eric M; Jones, Chloe M; Ramsay, Ian et al. (2016) A Janus Chelator Enables Biochemically Responsive MRI Contrast with Exceptional Dynamic Range. J Am Chem Soc 138:15861-15864

Showing the most recent 10 out of 25 publications