High-resolution structures of biological molecules obtained through x-ray crystallography provide among the most important sources of data for understanding function and mechanism. However, the process of crystallization presents a major bottleneck since hundreds or thousands of conditions need to be evaluated to find initial crystal "hits" that, through iterative rounds of optimization, become useful for structure determination. We propose to acquire a system for the automated optimization of crystallization experiments in order to expand the number of crystallization trials that can be effectively performed in a rapid and reproducible manner, thus to dramatically enhance the rate of success. Columbia University has a large and vibrant structural biology community, involved in numerous projects of substantial biomedical importance, and many members play leading role in NIH-funded high-throughput crystallography projects that will directly benefit from this instrument. After substantial due diligence, we have opted to propose the acquisition of an Eppendorf epMotion 5075 TMX liquid handling system for the automated generation of optimization screens to generate diffraction-quality crystals for structure determination.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD012012-01A1
Application #
8447754
Study Section
Special Emphasis Panel (ZRG1-BCMB-R (30))
Program Officer
Levy, Abraham
Project Start
2013-07-01
Project End
2014-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
1
Fiscal Year
2013
Total Cost
$120,673
Indirect Cost
Name
Columbia University (N.Y.)
Department
Physiology
Type
Schools of Medicine
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032