This proposal requests funding for a Q-Exactive Orbitrap LC-MS/MS system to be placed in the Mass Spectrometry Facility of the Department of Chemistry and Biochemistry at the University of Delaware. To maximize the utility of this """"""""workhorse"""""""" instrument, it will be configured with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources, as well as a conventional-flow LC with fast column- switching capabilities and a nano-LC for analysis of peptides. This instrument will provide research capabilities that are currently lacking at the University of Delaware (UD). Currently, researchers at UD have no on-site or in-state access to high-resolution accurate mass mass spectrometry (HRMS) instruments for characterization of small molecules. This instrument will provide this crucial tool to these researchers. Further, the Orbitrap LC- MS/MS will enable MS analysis of protein and peptide samples essential for many biomedical research programs at UD. The high sensitivity and broad coverage of the Orbitrap LC-MS/MS will be superior to that of the aging Q-ToF instrument, which is in continuous use by UD researchers. A well-defined management plan and high level of institutional commitment ensure the sustainability of this broadly used instrument. The proposed instrument will provide critical support for NIH-funded research programs of 6 major and 7 minor users spanning biochemistry, chemistry and materials science. The LC-MS/MS will greatly enable advances across a wide range of biomedical research areas, including structural enzymology, synthetic method development, protein assemblies, signaling and regulatory pathways for enzymes, molecular design, materials science for tissue regeneration and diagnostic devices, and chemical probe discovery. The instrument will also provide support to several major NIH initiatives at the University of Delaware, including the Chemistry-Biology Interface (CBI) Training Program, multiple Centers of Biomedical Research Excellence (COBRE) on Delaware's campus, the Delaware IDeA Network of Biomedical Research Excellence (INBRE), and the Center for Translational Cancer Research (CTCR).
The proposed Q-Exactive Orbitrap LC-MS/MS instrument is relevant to public health, because it will strongly support biomedical research efforts at the University of Delaware. The impacted research programs will advance the nation's health interests across a wide range of areas, including cancer, HIV, Crohn's disease, anaphylactic shock, asthma, atherosclerosis, disease detection, synthesis of bioactive molecules, in vivo imaging, nerve agent antidotes, and tissue regeneration. Further, the proposed instrument will support several major NIH initiatives at the University of Delaware, including the Chemistry-Biology Interface (CBI) Training Program, multiple Centers of Biomedical Research Excellence (COBRE), the Delaware IDeA Network of Biomedical Research Excellence (INBRE), and the Center for Translational Cancer Research (CTCR).
|Zhou, Qi; Cobb, Kelsey M; Tan, Tianyu et al. (2016) Stereospecific Cross Couplings To Set Benzylic, All-Carbon Quaternary Stereocenters in High Enantiopurity. J Am Chem Soc 138:12057-60|
|Fash, David M; Peer, Cody J; Li, Zhenwu et al. (2016) Synthesis of a stable and orally bioavailable englerin analogue. Bioorg Med Chem Lett 26:2641-4|
|Mori-Quiroz, Luis M; Shimkin, Kirk W; Rezazadeh, Sina et al. (2016) Copper-Catalyzed Amidation of Primary and Secondary Alkyl Boronic Esters. Chemistry 22:15654-15658|
|Basch, Corey H; Cobb, Kelsey M; Watson, Mary P (2016) Nickel-Catalyzed Borylation of Benzylic Ammonium Salts: Stereospecific Synthesis of Enantioenriched Benzylic Boronates. Org Lett 18:136-9|
|DeAngelis, Andrew; Panish, Robert; Fox, Joseph M (2016) Rh-Catalyzed Intermolecular Reactions of Î±-Alkyl-Î±-Diazo Carbonyl Compounds with Selectivity over Î²-Hydride Migration. Acc Chem Res 49:115-27|
|Zhou, Qi; Srinivas, Harathi D; Zhang, Songnan et al. (2016) Accessing Both Retention and Inversion Pathways in Stereospecific, Nickel-Catalyzed Miyaura Borylations of Allylic Pivalates. J Am Chem Soc 138:11989-95|
|Panish, Robert A; Chintala, Srinivasa R; Fox, Joseph M (2016) A Mixed-Ligand Chiral Rhodium(II) Catalyst Enables the Enantioselective Total Synthesis of Piperarborenineâ€…B. Angew Chem Int Ed Engl 55:4983-7|
|Dasgupta, Srimoyee; Liu, Jixin; Shoffler, Clarissa A et al. (2016) Enantioselective, Copper-Catalyzed Alkynylation of Ketimines To Deliver Isoquinolines with Î±-Diaryl Tetrasubstituted Stereocenters. Org Lett 18:6006-6009|
|Wu, Yue; Johnston, Murray V (2016) Molecular Characterization of Secondary Aerosol from Oxidation of Cyclic Methylsiloxanes. J Am Soc Mass Spectrom 27:402-9|
|Shimkin, Kirk W; Gildner, Peter G; Watson, Donald A (2016) Copper-Catalyzed Alkylation of Nitroalkanes with Î±-Bromonitriles: Synthesis of Î²-Cyanonitroalkanes. Org Lett 18:988-91|
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