Fluorescent microscopy has revolutionized modern biology. From calcium sensitive dyes that track the exquisite regulation of this second messenger to fluorescent fusion proteins that allow us to pinpoint the subcellular distribution of proteins, our ability to observe the inner workings of a cell is constantly expanding. To take advantage of the benefits of these advances we require state-of-the-art microscopy tools. Our current demand for confocal microscopy has exceeded our capacity and our current technology. We are requesting funding for a Zeiss LSM 710 confocal microscope to support our investigation of diverse aspects of developmental and cellular biology with biomedical applications. This microscope was selected as the most capable laser-scanning microscope that can support the research projects of our users, which include biologists with developmental, cellular, and tissue engineering research areas. Moreover, this microscope is modular and expandable to support future applications, including those not yet envisioned. The LSM 710 has incorporated several improvements over previous Zeiss versions, including higher sensitivity, reduced background noise, improved flexibility for new fluorochromes, and integrated analytical methods and is thus ideally suited for the needs of our expanding group of NIH- funded investigators at Rice University.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR026399-01
Application #
7791586
Study Section
Special Emphasis Panel (ZRG1-IMST-A (30))
Program Officer
Levy, Abraham
Project Start
2010-05-13
Project End
2011-05-12
Budget Start
2010-05-13
Budget End
2011-05-12
Support Year
1
Fiscal Year
2010
Total Cost
$500,000
Indirect Cost
Name
Rice University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
050299031
City
Houston
State
TX
Country
United States
Zip Code
77005
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Gonzalez, Kim L; Fleming, Wendell A; Kao, Yun-Ting et al. (2017) Disparate peroxisome-related defects in Arabidopsis pex6 and pex26 mutants link peroxisomal retrotranslocation and oil body utilization. Plant J 92:110-128
Rinaldi, Mauro A; Patel, Ashish B; Park, Jaeseok et al. (2016) The Roles of ?-Oxidation and Cofactor Homeostasis in Peroxisome Distribution and Function in Arabidopsis thaliana. Genetics 204:1089-1115
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Burkhart, Sarah E; Kao, Yun-Ting; Bartel, Bonnie (2014) Peroxisomal ubiquitin-protein ligases peroxin2 and peroxin10 have distinct but synergistic roles in matrix protein import and peroxin5 retrotranslocation in Arabidopsis. Plant Physiol 166:1329-44
Lukianova-Hleb, Ekaterina Y; Ren, Xiaoyang; Sawant, Rupa R et al. (2014) On-demand intracellular amplification of chemoradiation with cancer-specific plasmonic nanobubbles. Nat Med 20:778-784
Lukianova-Hleb, Ekaterina Y; Lapotko, Dmitri O (2014) Malaria theranostics using hemozoin-generated vapor nanobubbles. Theranostics 4:761-9
Woodward, Andrew W; Fleming, Wendell A; Burkhart, Sarah E et al. (2014) A viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes. Plant Mol Biol 86:201-14

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