The Gladstone Flow Core is requesting funds to purchase a Becton Dickinson FACSAria II specifically customized for research at the Gladstone Institutes. This cell sorter is equipped with optional yellow-green, UV, and high-powered violet lasers, an automatic cell deposition unit, and a temperature control. Each of these options was carefully considered and responds to the need of one of the nine NIH-funded studies from seven Gladstone investigators. These projects are centered on a broad range of basic research in relation to cardiovascular and infectious diseases. Marielle Cavrois, the Director of the Gladstone Flow Core, will administer the instrument and oversee its operation by our highly trained technician. The FACSAria II was selected for its fixed optical system, a feature that simplifies calibration and maintenance, and will permit the major users to self-operate the instrument after training by the Gladstone Flow Core staff, extending the hours of operation for the benefit of our major users group. The FACSAria II possesses an aerosol management option and will be sited in the Gladstone BSL-3 laboratory to ensure safe cell sorting of samples contaminated with blood-borne pathogens. This unique feature, coupled with the multiple laser options, will be highly valuable to the wider research community on the growing Mission Bay campus, to whom the remaining time not allocated to our major users group can be made available. The FACSAria II flow cytometer is essential Gladstone's basic research on three of the most important medical problems of modern times: cardiovascular disease, HIV/AIDS and Alzheimer's disease.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR028962-01
Application #
7840236
Study Section
Special Emphasis Panel (ZRG1-CB-P (30))
Program Officer
Levy, Abraham
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
1
Fiscal Year
2011
Total Cost
$544,471
Indirect Cost
Name
J. David Gladstone Institutes
Department
Type
DUNS #
099992430
City
San Francisco
State
CA
Country
United States
Zip Code
94158
Kime, Cody; Sakaki-Yumoto, Masayo; Goodrich, Leeanne et al. (2016) Autotaxin-mediated lipid signaling intersects with LIF and BMP signaling to promote the naive pluripotency transcription factor program. Proc Natl Acad Sci U S A 113:12478-12483
Muñoz-Arias, Isa; Doitsh, Gilad; Yang, Zhiyuan et al. (2015) Blood-Derived CD4 T Cells Naturally Resist Pyroptosis during Abortive HIV-1 Infection. Cell Host Microbe 18:463-70
Razooky, Brandon S; Pai, Anand; Aull, Katherine et al. (2015) A hardwired HIV latency program. Cell 160:990-1001