Ctytochrome P450 inhibitors selective for certain P450 enzymes (1A1,1A2, 2A6, and 2B1) could potentially be used as anti-initiation agents and as agents used to modify stages of cancer promotion and progression. This study is designed to """"""""map"""""""" the three-dimensional structure and electronic character of a series of aromatic acetylenes known to act as mechanism based inhibitors of cytochrome P450 subtypes 1A1, 1A2, 2A6, and 2B1. The mapping will include the overall geometry of the molecules as determined by X-ray crystallographic techniques. It will also include the electronic characteristics of the acetylene groups determined by net atomic charges, electron density distributions, and electrostatic potentials. In addition, this project will include a molecular modeling study of a large series of aromatic acetylenes known to act as inhibitors of P450 enzymes. This study will include determination of the 3D-QSAR and a pharmacophore map. Docking of these inhibitors into the active sites of the crystal structures of P450s 1A2 and 2A6, as well as homology models of P450s 1A1 and 2B1, will be used to explore the mechanism of P450 inhibition by aromatic acetylenes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Enhancement Award (SC1)
Project #
5SC1GM084722-04
Application #
8116534
Study Section
Special Emphasis Panel (ZGM1-MBRS-1 (CH))
Program Officer
Okita, Richard T
Project Start
2008-08-01
Project End
2012-07-31
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
4
Fiscal Year
2011
Total Cost
$205,821
Indirect Cost
Name
Xavier University of Louisiana
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
020857876
City
New Orleans
State
LA
Country
United States
Zip Code
70125
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Liu, Jiawang; Nguyen, Thong T; Dupart, Patrick S et al. (2012) 7-Ethynylcoumarins: selective inhibitors of human cytochrome P450s 1A1 and 1A2. Chem Res Toxicol 25:1047-57
Sridhar, Jayalakshmi; Ellis, Jamie; Dupart, Patrick et al. (2012) Development of flavone propargyl ethers as potent and selective inhibitors of cytochrome P450 enzymes 1A1 and 1A2. Drug Metab Lett 6:275-84
Zhu, Naijue; Lightsey, Danielle; Liu, Jiawang et al. (2010) Ethynyl and Propynylpyrene Inhibitors of Cytochrome P450. J Chem Crystallogr 40:343-352
Sridhar, Jayalakshmi; Jin, Ping; Liu, Jiawang et al. (2010) In silico studies of polyaromatic hydrocarbon inhibitors of cytochrome P450 enzymes 1A1, 1A2, 2A6, and 2B1. Chem Res Toxicol 23:600-7