Bacterial meningitis is a severe bacterial infection of the protective brain membranes and can become fatal in as early as 24 hours after symptoms are noticed. According to the World Health Organization, one million cases of bacterial meningitis are estimated to occur and 200,000 of these die annually. However, current approaches for meningitis diagnosis are either costly and time consuming (e.g. cell culture, which may take more than 48 hours.), or need expensive specialized equipment available only in laboratories (e.g. thermal cyclers needed for real-time PCR). All these factors limit the applications of those approaches in resource-limited settings. Considering that many cases of meningitis cases happened in rural high-poverty areas, and the high fatality rate of meningitis, a simple, low-cost, highly-sensitive device and method are greatly needed for immediate and early diagnosis of meningitis in resource-poor settings. The goal of this proposal is to develop a low-cost point of care (POC) device for rapid and highly-sensitive diagnosis of three different types of bacterial meningitis, namely Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumonia especially for resource-poor settings. DNA codes with different DNA sequences to target these pathogens will be grafted to paper by utilizing the interaction between the nanomaterial graphene oxide (GO) and single strand DNA. Multiple capture zones on microfluidic devices allow for the detection of these three different species of bacteria on chip simultaneously and specifically, based on DNA hybridization with specific DNA capture probes. A miniaturized film heater will be devised to integrate the loop-mediated isothermal amplification (LAMP) on chip, providing high detection sensitivity. In addition, we also aim to design and perfect an instrument-free, paper-based device for multiplex meningitis diagnosis in field. This project will provide a low-cost paper-based microfluidic POC device and method for immediate and early diagnosis of meningitis especially in resource-poor settings. It will also have great potential in the detection of various plant, animal, foodborne, and infectious diseases from other microorganisms.
Bacterial meningitis is a severe bacterial infection of the protective brain membranes and can become fatal in as early as 24 hours after symptoms are noticed. Current approaches for meningitis diagnosis, however, are either costly and time consuming, or need expensive specialized equipment available only in laboratories, limiting the applications of those approaches in low-resource settings. We are developing a low-cost point of care (POC) biochip based on DNA testing for fast and early diagnosis of three different types of meningitis simultaneously especially for resource-poor settings.
|Dou, Maowei; Sanjay, Sharma T; Dominguez, Delfina C et al. (2017) Multiplexed instrument-free meningitis diagnosis on a polymer/paper hybrid microfluidic biochip. Biosens Bioelectron 87:865-873|
|Dou, Maowei; Sanjay, Sharma T; Dominguez, Delfina C et al. (2017) A paper/polymer hybrid CD-like microfluidic SpinChip integrated with DNA-functionalized graphene oxide nanosensors for multiplex qLAMP detection. Chem Commun (Camb) 53:10886-10889|
|Shen, Feng; Li, Yi; Liu, Zhaomiao et al. (2017) Study of flow behaviors of droplet merging and splitting in microchannels using Micro-PIV measurement. Microfluid Nanofluidics 21:|
|Zhan, Sihui; Zhang, He; Zhang, Yu et al. (2017) Efficient NH3-SCR removal of NOx with highly ordered mesoporous WO3(?)-CeO2 at low temperatures. Appl Catal B 203:199-209|
|Sanjay, Sharma T; Zhou, Wan; Dou, Maowei et al. (2017) Recent advances of controlled drug delivery using microfluidic platforms. Adv Drug Deliv Rev :|
|Sanjay, Sharma T; Dou, Maowei; Sun, Jianjun et al. (2016) A paper/polymer hybrid microfluidic microplate for rapid quantitative detection of multiple disease biomarkers. Sci Rep 6:30474|
|Fu, Guanglei; Sanjay, Sharma T; Dou, Maowei et al. (2016) Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer. Nanoscale 8:5422-7|
|Sanjay, Sharma T; Dou, Maowei; Fu, Guanglei et al. (2016) Controlled Drug Delivery Using Microdevices. Curr Pharm Biotechnol 17:772-87|
|Dou, Maowei; Lopez, Juan; Rios, Misael et al. (2016) A fully battery-powered inexpensive spectrophotometric system for high-sensitivity point-of-care analysis on a microfluidic chip. Analyst 141:3898-903|
|Dou, Maowei; García Jr, José Mireles; Zhan, Sihui et al. (2016) Interfacial nano-biosensing in microfluidic droplets for high-sensitivity detection of low-solubility molecules. Chem Commun (Camb) 52:3470-3|
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