(largely taken from the application) This program provides training to chemists, biochemists, biophysicists, and biologists in hemoglobin, blood proteins, and related protein chemical studies. A faculty of 18 scientists has been assembled to provide the training. The faculty are actively engaged in studies of electron transfer within proteins, oxygen binding to hemoproteins, the structure of membrane-embedded proteins, the structure of proteins involved in regulatory processes, cellular immunology, transcriptional regulation, and clotting processes. The faculty provides training in electron spin resonance, ultrafast kinetics, x-ray crystallography, nuclear magnetic resonance spectroscopy, fluorescence energy transfer, protein chemistry, site-directed mutations, cell culture, immunochemistry, gene cloning, and the expression of cloned proteins. Trainees have the opportunity to receive training in this wide array of techniques and to apply them to specific problems in biomedical research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Institutional National Research Service Award (T32)
Project #
5T32DK007233-23
Application #
2770307
Study Section
Special Emphasis Panel (SRC)
Program Officer
Bishop, Terry Rogers
Project Start
1976-07-01
Project End
2001-08-31
Budget Start
1998-09-15
Budget End
1999-08-31
Support Year
23
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Wilderman, P Ross; Jang, Hyun-Hee; Malenke, Jael R et al. (2014) Functional characterization of cytochromes P450 2B from the desert woodrat Neotoma lepida. Toxicol Appl Pharmacol 274:393-401
Baxter, Elizabeth Leigh; Zuris, John A; Wang, Charles et al. (2013) Allosteric control in a metalloprotein dramatically alters function. Proc Natl Acad Sci U S A 110:948-53
Tamir, Sagi; Zuris, John A; Agranat, Lily et al. (2013) Nutrient-deprivation autophagy factor-1 (NAF-1): biochemical properties of a novel cellular target for anti-diabetic drugs. PLoS One 8:e61202
Wilderman, P Ross; Gay, Sean C; Jang, Hyun-Hee et al. (2012) Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme. FEBS J 279:1607-20
Kang, Guipeun; López-Peña, Ignacio; Oklejas, Vanessa et al. (2012) Förster resonance energy transfer as a probe of membrane protein folding. Biochim Biophys Acta 1818:154-61
Wilderman, P Ross; Halpert, James R (2012) Plasticity of CYP2B enzymes: structural and solution biophysical methods. Curr Drug Metab 13:167-76
Wu-Zhang, Alyssa X; Schramm, Cicely L; Nabavi, Sadegh et al. (2012) Cellular pharmacology of protein kinase M? (PKM?) contrasts with its in vitro profile: implications for PKM? as a mediator of memory. J Biol Chem 287:12879-85
Olson, Karen E; Muller, Ulrich F (2012) An in vivo selection method to optimize trans-splicing ribozymes. RNA 18:581-9
Nechushtai, Rachel; Conlan, Andrea R; Harir, Yael et al. (2012) Characterization of Arabidopsis NEET reveals an ancient role for NEET proteins in iron metabolism. Plant Cell 24:2139-54
Meluzzi, Dario; Olson, Karen E; Dolan, Gregory F et al. (2012) Computational prediction of efficient splice sites for trans-splicing ribozymes. RNA 18:590-602

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