The Program in Molecular Biophysics (PMB) seeks to renew its training grant. Its goal is to train students thoroughly versed in the fundamental physical principles governing the structures, interactions, and functions of biological macromolecules and their complexes, and who are also cognizant of the current frontiers in biology and able to develop their research in biologically relevant terms. The Department of Biophysics (Arts &Sciences, Homewood campus) and the Department of Biophysics &Biophysical Chemistry (School of Medicine, East Baltimore campus) form the program core with respect to administration and curriculum;42 faculty from 14 departments participate in training activities. The participating laboratories form a coherent group with a strong sense of community engendered by activities such as an annual retreat and a monthly evening Biophysical Discussion. Faculty and students also have access to shared instrumentation facilities for X-ray, ultracentrifuge, high field NMR, and mass spectrometry. Students freely move between campuses and laboratories for course work, rotations, and thesis research. Students with strong backgrounds in quantitative and physical sciences are recruited;most are familiar with biochemistry and molecular biology. In the first year, students take four core courses specifically designed for PMB;they cover thermodynamic and structural principles of molecular biophysics as well as relevant computational and experimental methods. Readings from the literature and computer programming exercises are extensively incorporated into class assignments, and the courses emphasize quantitative analysis and fundamental physical principles. First year students also complete three 10 week lab rotations and a lecture series on Responsible Conduct of Research. Students choose a lab for thesis research at the end of the second semester, and also must demonstrate proficiency in biochemistry and molecular and cellular biology in an oral exam. Second year students take Organic Mechanisms in Biology and two electives;present a formal seminar on a current topic in biophysics and take an oral qualifying exam. 16 trainees are currently supported by this grant, out of a total of 50 program students.

Public Health Relevance

Living cells and organisms ultimately depend on the complex interactions taking place between thousands of proteins, nucleic acids, and smaller molecules. In this training program, students learn to use the methods of physical sciences and computation to investigate this network of interactions, and how the molecular details of these interactions illuminate the underlying causes of specific diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Institutional National Research Service Award (T32)
Project #
5T32GM008403-23
Application #
8299045
Study Section
National Institute of General Medical Sciences Initial Review Group (BRT)
Program Officer
Flicker, Paula F
Project Start
1990-09-30
Project End
2015-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
23
Fiscal Year
2012
Total Cost
$714,473
Indirect Cost
$33,961
Name
Johns Hopkins University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Plummer, Ashlee M; Fleming, Karen G (2016) From Chaperones to the Membrane with a BAM! Trends Biochem Sci 41:872-82
Klein, Max; Sharma, Rati; Bohrer, Chris H et al. (2016) Biospark: scalable analysis of large numerical datasets from biological simulations and experiments using Hadoop and Spark. Bioinformatics :
McDonald, Sarah K; Fleming, Karen G (2016) Aromatic Side Chain Water-to-Lipid Transfer Free Energies Show a Depth Dependence across the Membrane Normal. J Am Chem Soc 138:7946-50
Morgan, Michael T; Haj-Yahya, Mahmood; Ringel, Alison E et al. (2016) Structural basis for histone H2B deubiquitination by the SAGA DUB module. Science 351:725-8
DiBello, Anthony; Datta, Ajit B; Zhang, Xiangbin et al. (2016) Role of E2-RING Interactions in Governing RNF4-Mediated Substrate Ubiquitination. J Mol Biol 428:4639-4650
Moreno, Andrew; Froehlig, John R; Bachas, Sharrol et al. (2016) Solution Binding and Structural Analyses Reveal Potential Multidrug Resistance Functions for SAV2435 and CTR107 and Other GyrI-like Proteins. Biochemistry 55:4850-63
Motlagh, Hesam N; Toptygin, Dmitri; Kaiser, Christian M et al. (2016) Single-Molecule Chemo-Mechanical Spectroscopy Provides Structural Identity of Folding Intermediates. Biophys J 110:1280-90
Geiger-Schuller, Kathryn; Barrick, Doug (2016) Broken TALEs: Transcription Activator-like Effectors Populate Partly Folded States. Biophys J 111:2395-2403
Zaccai, Nathan R; Sandlin, Clifford W; Hoopes, James T et al. (2016) Deuterium Labeling Together with Contrast Variation Small-Angle Neutron Scattering Suggests How Skp Captures and Releases Unfolded Outer Membrane Proteins. Methods Enzymol 566:159-210
Coltharp, Carla; Buss, Jackson; Plumer, Trevor M et al. (2016) Defining the rate-limiting processes of bacterial cytokinesis. Proc Natl Acad Sci U S A 113:E1044-53

Showing the most recent 10 out of 71 publications