We describe the continuation of a pre-doctoral training program in Cellular, Biochemical and Molecular Biology (http://dbb.urmc.rochester.edu/T32/index.htm) with a core of interactive investigators in RNA, DNA, and protein biochemistry. The proposed training program capitalizes on (i) initiatives that expand research and education at the University of Rochester Medical Center (URMC) and College of Arts and Sciences (CAS), and (ii) enhanced educational choices resulting from the successful reorganization of Graduate Education in Biomedical Sciences (GEBS). Students are accepted into multidisciplinary "Clusters". They experience diverse research areas in their first year through courses, faculty research presentations, and laboratory rotations. They then choose a research advisor and degree program from a wide range of opportunities. We will maintain successful T32 initiatives: 1) provide one year of financial support for 6-8 students performing interdisciplinary research;2) promote direct interactions between students and visiting seminar speakers;3) offer practice in critical thinking in research science;4) provide opportunities for off-site student travel to attend meetings, take courses, and work in outside laboratories;5) counsel female and minority students to achieve career goals;6) foster interactions among investigators and students in the areas of RNA, DNA, and protein biochemistry through course requirements and a yearly retreat;and 7) augment effective student recruiting through training-grant educational benefits. The University is benefiting from the recent appointments of a new President and Medical Center Director. Since funding of this T32, the URMC has not only filled two new research facilities (400,000 sq. ft.) and started new programs in Genetics, Biochemistry, Structural Biology, Virology, Signaling, Immunology, Neurobiology and Stem Cells, increasing the number of PIs from 150 to about 250, but also initiated a Strategic Plan with the construction of 500,000 sq. ft. of new labs. The Strategic Plan includes creation of five Innovative Science Programs that increase the opportunities for graduate training. Within this expanding infrastructure, our T32 continues to promote interactions among labs, flexibility in the choice of research mentors, and improved graduate recruiting.

Public Health Relevance

Research funded by this T32 pertains to cellular, biochemical and molecular biology, with a focus on the structure and function of DNA, RNA and proteins. In view of the recent appreciation that most of genomic DNA is transcribed into RNA and that many genes encode more than one protein, research supported by this T32 will lend important insight into the mechanisms of cellular maintenance, growth and differentiation.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Institutional National Research Service Award (T32)
Project #
Application #
Study Section
National Institute of General Medical Sciences Initial Review Group (BRT)
Program Officer
Gindhart, Joseph G
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Rochester
Schools of Dentistry
United States
Zip Code
Wakabayashi, Hironao; Monaghan, Morgan; Fay, Philip J (2014) Cofactor activity in factor VIIIa of the blood clotting pathway is stabilized by an interdomain bond between His281 and Ser524 formed in factor VIII. J Biol Chem 289:14020-9
Monaghan, M; Wakabayashi, H; Griffiths, A et al. (2014) Enhanced factor VIIIa stability of A2 domain interface variants results from an increased apparent affinity for the A2 subunit. Results from an increased apparent affinity for the A2 subunit. Thromb Haemost 112:495-502
Cadiz-Rivera, Brenda; Fromm, George; de Vries, Christina et al. (2014) The chromatin "landscape" of a murine adult ?-globin gene is unaffected by deletion of either the gene promoter or a downstream enhancer. PLoS One 9:e92947
Ortiz-Riano, Emilio; Ngo, Nhi; Devito, Stefanie et al. (2014) Inhibition of arenavirus by A3, a pyrimidine biosynthesis inhibitor. J Virol 88:878-89
Pepenella, Sharon; Murphy, Kevin J; Hayes, Jeffrey J (2014) A distinct switch in interactions of the histone H4 tail domain upon salt-dependent folding of nucleosome arrays. J Biol Chem 289:27342-51
Leioatts, Nicholas; Mertz, Blake; Martinez-Mayorga, Karina et al. (2014) Retinal ligand mobility explains internal hydration and reconciles active rhodopsin structures. Biochemistry 53:376-85
Leioatts, Nicholas; Suresh, Pooja; Romo, Tod D et al. (2014) Structure-based simulations reveal concerted dynamics of GPCR activation. Proteins 82:2538-51
Ofori, Leslie O; Hilimire, Thomas A; Bennett, Ryan P et al. (2014) High-affinity recognition of HIV-1 frameshift-stimulating RNA alters frameshifting in vitro and interferes with HIV-1 infectivity. J Med Chem 57:723-32
Guy, Michael P; Young, David L; Payea, Matthew J et al. (2014) Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis. Genes Dev 28:1721-32
Pepenella, Sharon; Murphy, Kevin J; Hayes, Jeffrey J (2014) Intra- and inter-nucleosome interactions of the core histone tail domains in higher-order chromatin structure. Chromosoma 123:3-13

Showing the most recent 10 out of 57 publications