We have recently developed a prototype system for long-term monitoring of single-cell behaviors in complex tissues. Our system comes from the synergistic integration and optimization of two leading technologies in microscopy and computational cell tracking: the inverted selective plane illumination microscope (iSPIM) and the StarryNite software package. We have demonstrated order-of-magnitude improvements on virtually all fronts compared to the standard technologies in use, notably imaging speed, reduction of phototoxicity, accuracy of cell tracking, and lower demand for computing power, all achieved at a reduced cost with comparable or better image quality. Our system has been applied to multiple model organisms as well as cell culture, demonstrating its versatility.
We aim to bring the system to maturity and into the hands of the research community as a powerful and versatile tool for single-cell studies in complex, differentiating cell populations.

Public Health Relevance

Long-term monitoring of single cells in the context of complex tissues is crucial for the understanding of cell biological changes in physiological contexts and disease. Here we propose to develop a mature system to monitor individual cells in complex tissues based on a novel microscopy and computer-based automated image analysis system. Our system will provide a powerful tool to enable the research community to achieve better understanding on a wide range of health relative questions at unprecedented temporal and spatial resolution, from birth defects to brain function and viral infection.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01HD075602-03
Application #
8704126
Study Section
Special Emphasis Panel (ZRG1-CB-D (50))
Program Officer
Mukhopadhyay, Mahua
Project Start
2012-09-24
Project End
2017-07-31
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
3
Fiscal Year
2014
Total Cost
$651,664
Indirect Cost
$145,688
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
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Du, Zhuo; Santella, Anthony; He, Fei et al. (2014) De novo inference of systems-level mechanistic models of development from live-imaging-based phenotype analysis. Cell 156:359-72
Wu, Yicong; Wawrzusin, Peter; Senseney, Justin et al. (2013) Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy. Nat Biotechnol 31:1032-8