The Midwest Progenitor Ciell Consortium is a collaboration between the UW-Madison and the UMN which will serve as a research hub within the NHLBI's Progenitor Cell Biology Consortium. The goal is to develop new strategies to convert human pluripotent stem and somatic cells into hematopoietic stem cells (HSCs) and selfrenewing cardiac progenitor cells through identification of genetic program leading to formation of pre-HSCs and cardiac progenitors and activation of self-renewal program in lineage-restricted cells. Project 1, """"""""The De Novo Generation of Hematopoietic Stem CeUs from Human ES/iPS Cells and Somatic Cells,"""""""" aims are: Identify the hierarchy of mesodermal progenitors of vascular endothelial and hematopoietic lineages using human ES cell lines with targeted FOXFl, GATA-2, GATA-3, RUNXl, and SCL genes;Determine the most critical molecular events leading to formation of hematopoietic cells and HSCs during embryogenesis in mouse aind human, and following in vitro differentiation of human pluripotent stem cells;Develop technologies for de novo generation of HSCs from human ES/iPS cells and somatic cells. Project 2, """"""""Cardiovascular Progenitors and Cardiomyocytes from Human ES/iPS Cells and Somatic Cells,"""""""" aims are: Isolate and define human embryonic cardiovascular progenitors and ventricular myocytes derived from pluripotent stem cells;and reprogram human pluripotent stem cells and somatic cells using transcription factors to generate cardiovascular progenitors and ventricular myocytes. Project 3, """"""""Artificial Transcription Factors for Reprogramming/Transdifferentation,"""""""" aims are: Design ATFs that activate expression of Oct4, Sox2 or Nanog in primary fibroblasts;Perform genome-wide location analysis, CSI and transcriptome analysis to determine gene targets and specificity ofthe designed ATFs;and combine ATFs to induce pluripotency in primary fibroblasts. Project 4, """"""""Rapid In Vitro Generation of Affinity Reagents for Hematopoietic and Cardiovascular Precursors,"""""""" aims are: Develop methods for rapidly generating aptamers that bind to transmembrane protein targets with high affinity and specificity;Develop methods to accelerate discovery of aptamers with sub nanomolar affinities using high-throughput DNA sequencing technologies;and generate high affinity aptamers for surface markers of cardiovascular and blood precursors.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Project--Cooperative Agreements (U01)
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Special Emphasis Panel (ZHL1-CSR-J (S1))
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Thomas, John
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Morgridge Institute for Research, Inc.
United States
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Bacher, Rhonda; Leng, Ning; Chu, Li-Fang et al. (2018) Trendy: segmented regression analysis of expression dynamics in high-throughput ordered profiling experiments. BMC Bioinformatics 19:380
Kang, HyunJun; Mesquitta, Walatta-Tseyon; Jung, Ho Sun et al. (2018) GATA2 Is Dispensable for Specification of Hemogenic Endothelium but Promotes Endothelial-to-Hematopoietic Transition. Stem Cell Reports 11:197-211
Heiderscheit, Evan A; Eguchi, Asuka; Spurgat, Mackenzie C et al. (2018) Reprogramming cell fate with artificial transcription factors. FEBS Lett 592:888-900
Slukvin, Igor I; Kumar, Akhilesh (2018) The mesenchymoangioblast, mesodermal precursor for mesenchymal and endothelial cells. Cell Mol Life Sci :
Park, Mi Ae; Jung, Ho Sun; Slukvin, Igor (2018) Genetic Engineering of Human Pluripotent Stem Cells Using PiggyBac Transposon System. Curr Protoc Stem Cell Biol 47:e63
Kumar, Akhilesh; D'Souza, Saritha Sandra; Moskvin, Oleg V et al. (2017) Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts. Cell Rep 19:1902-1916
Bylund, Jeffery B; Trinh, Linh T; Awgulewitsch, Cassandra P et al. (2017) Coordinated Proliferation and Differentiation of Human-Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Depend on Bone Morphogenetic Protein Signaling Regulation by GREMLIN 2. Stem Cells Dev 26:678-693
Wang, Jinpeng; Yu, Jingwen; Yang, Qin et al. (2017) Multiparameter Particle Display (MPPD): A Quantitative Screening Method for the Discovery of Highly Specific Aptamers. Angew Chem Int Ed Engl 56:744-747
Rodríguez-Martínez, José A; Reinke, Aaron W; Bhimsaria, Devesh et al. (2017) Combinatorial bZIP dimers display complex DNA-binding specificity landscapes. Elife 6:
Jiang, Peng; Hou, Zhonggang; Bolin, Jennifer M et al. (2017) RNA-Seq of Human Neural Progenitor Cells Exposed to Lead (Pb) Reveals Transcriptome Dynamics, Splicing Alterations and Disease Risk Associations. Toxicol Sci 159:251-265

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