Monitoring for pathogens causing enteric diseases such as salmonellosis is needed in a number of sectors including food and feed production, retail facilities, and veterinary hospitals. Several commercially available kits are available for this purpose, but are validated primarily for human food testing. A comprehensive monitoring plan for food production facilities should include the ability to test multiple types of samples, including tissues and feces from sick or dead animals and/or rodents and environmental swabs. Validation of rapid assays for additional sample types such as animal tissues, fecal samples, and environmental swabs is urgently needed as part of comprehensive surveillance efforts and outbreak management plans. There is a large demand for rapid screening for enteric bacterial pathogens such as Salmonella spp. in a number of sectors including food and feed production facilities. Several commercially available kits are available for this purpose, but are AOAC validated only for human food testing. Validation of rapid assays for additional matrices such as animal tissues, fecal samples, and environmental surface swabs is urgently needed. In order to accomplish this, we propose the following specific aims: 1) Validate commercially available molecular diagnostic assays for Salmonella spp., E. coli, and Listeria spp. for use in fecal samples, animal tissues, and environmental swabs;2) Transfer these assays to an open array platform in order to facilitate testing efficiency and transferability to other laboratories. Extending validations of rapid food testing kits for additional animal-related matrices, which is feasible due to our expertise in veterinary diagnostics, will have direct relevance to public health by providing new methods for comprehensive enteric pathogen surveillance and outbreak management. The long-term goal of the proposed research is to facilitate the dissemination of high-throughput, cost-effective methods for screening of multiple sample types for a broad range of enteric pathogens.

Public Health Relevance

The concept of One Health-One Medicine and the realization that the health of humans, animals and the environment are intertwined are of major importance for food safety programs. Since cross-contamination of food and feed can occur at many stages between the farm and the table, testing should not just be limited to the food product. This project will validate molecular bacteria food testing kits for veterinary and environmental samples and develop an enteric disease rapid surveillance panel for improving testing efficiency and coordination between diagnostic labs.

National Institute of Health (NIH)
Food and Drug Administration (FDA)
Research Demonstration--Cooperative Agreements (U18)
Project #
Application #
Study Section
Special Emphasis Panel (ZFD1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Cornell University
Veterinary Sciences
Schools of Veterinary Medicine
United States
Zip Code
Voorhees, Ian E H; Dalziel, Benjamin D; Glaser, Amy et al. (2018) Multiple Incursions and Recurrent Epidemic Fade-Out of H3N2 Canine Influenza A Virus in the United States. J Virol 92:
Goodman, Laura B; McDonough, Patrick L; Anderson, Renee R et al. (2017) Detection of Salmonella spp. in veterinary samples by combining selective enrichment and real-time PCR. J Vet Diagn Invest 29:844-851
Goodman, Laura B; Lawton, Marie R; Franklin-Guild, Rebecca J et al. (2017) Lactococcus petauri sp. nov., isolated from an abscess of a sugar glider. Int J Syst Evol Microbiol 67:4397-4404
Goodman, Laura B; Anderson, Renee R; Slater, Marcia et al. (2016) High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR. J Vis Exp :