The flow cytometry core will be an integral part of the overall proposal and will provide a facility for analyses of cell samples for surface markers associated with cell activation and function for assessment of purity of cell preparations used in functional assays for monitoring cell cycle progression for measuring apoptosis, and for determining cytokine synthesis by individual cells. In addition, the core will provide capabilities for sorting phenotypically distinct populations of cells for the projects which will investigate activities of heterogeneous lymphoid preparations. the core will be located at the La Jolla Institute for Allergy and Immunology, which is in walking distance from Scripps Research Institute, and will consist of several Becton Dickinson instruments including one FACScan used for analysis only, one FACScan and FACStar can distinguish fluorescence at three wavelengths simultaneously whereas the FACScaliber is capable of four color analyses. All instruments are available at the present time. Funds are requested to support the maintenance and repair of the instruments to insure they are functional and available to investigators at all times, and for part time support of a research technicians salary. The technician will oversee the daily running of the machines and provide assistance with analysis of samples. All projects of this program will utilize this core to some extent with project 3 heavily dependent on this facility (Project 1, 15%; Project 3, 65%, Project 4, 5%). FACS analyses will be used primarily to characterize various mast cell populations generated in Project 3, and also used in Projects I and 2, for surface markers such as B7-1 and -2, ICAM- 1, CD40- and CD40L, VLA-4 and -5, and for analyzing and purifying different subsets of T cells (Projects 1 and 3). Additional uses will include assessing C3a receptor expression, actin polymerization, and apoptosis (Project 2), calcium mobilization (Project 1) and bradykinin receptor expression (Project 4).

Project Start
1999-09-01
Project End
2000-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
La Jolla Institute
Department
Type
DUNS #
603880287
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Gong, Jian; Yang, Ning-Sun; Croft, Michael et al. (2010) The antigen presentation function of bone marrow-derived mast cells is spatiotemporally restricted to a subset expressing high levels of cell surface FcepsilonRI and MHC II. BMC Immunol 11:34
Chen, Swey-Shen; Gong, Jian; Yang, Yong-Min et al. (2005) Cytotoxic T-cells specific for natural IgE peptides downregulate IgE production. Cell Immunol 233:11-22
Gong, Jian; Liu, Fu-Tong; Chen, Swey-Shen (2004) Polyphenolic antioxidants enhance IgE production. Immunol Invest 33:295-307
Gong, Jian; Chen, Swey-Shen (2003) Polyphenolic antioxidants inhibit peptide presentation by antigen-presenting cells. Int Immunopharmacol 3:1841-52
Mayr, Susanne I; Zuberi, Riaz I; Zhang, Min et al. (2002) IgE-dependent mast cell activation potentiates airway responses in murine asthma models. J Immunol 169:2061-8
Chen, Huan Yuan; Liu, Fu-Tong; Hou, Charlie M H et al. (2002) Monoclonal antibodies against the C(epsilon)mX domain of human membrane-bound IgE and their potential use for targeting IgE-expressing B cells. Int Arch Allergy Immunol 128:315-24
Christiansen, Sandra C; Zuraw, Bruce L (2002) Serving the underserved: school-based asthma intervention programs. J Asthma 39:463-72
Asai, K; Kitaura, J; Kawakami, Y et al. (2001) Regulation of mast cell survival by IgE. Immunity 14:791-800
Field, K A; Apgar, J R; Hong-Geller, E et al. (2000) Mutant RBL mast cells defective in Fc epsilon RI signaling and lipid raft biosynthesis are reconstituted by activated Rho-family GTPases. Mol Biol Cell 11:3661-73
Kawakami, Y; Kitaura, J; Satterthwaite, A B et al. (2000) Redundant and opposing functions of two tyrosine kinases, Btk and Lyn, in mast cell activation. J Immunol 165:1210-9

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