Sample Core will be in charge of the collection, isolation of specific cells, and dispatching cell samples. The type of samples will contain 1) blood draw, 2) blood apheresis, and 3) nasal wash samples. In particular, all the samples obtained at the Clinical Core will be transferred to the Sample Core, and stored after cell isolation, if required. The Core also will perform cell phenotyping by polychromatic flow cytometry and the gene expression profiling of isolated cells in collaboration with the Microarray Core. The obtained data at the Core will be put in the database, where all the Projects will have access. The primary objectives of the Sample Core are: 1) To collect blood draw samples and apheresis samples from healthy subjects including those mobilized with G-CSF. 2) To elutriate cells from blood apheresis samples to obtain lymphocyte- and monocyte-rich fractions. 3) To isolate CD34+ hematopoietic progenitor cells from G-CSF mobilized apheresis blood samples. 4) To analyze the phenotype of blood and nasal wash samples by polychromatic flow cytometry. 5) To isolate specific cells by cell sorting from blood and nasal wash samples. 6) To dispatch elutriated or isolated cells to Projects and Tech Devs. Thus, the Sample Core will be a central facility to provide appropriate types of cells and data (including phenotype and microarray data) to all the Projects and Tech Devs.

Public Health Relevance

Sample Core will be in charge of the collection, isolation of specific cells, and dispatching cell samples. The Core also will perform immunological and gene analysis of isolated cells, which will be utilized in the Projects. Thus, the Sample Core will be a central facility to provide appropriate cells and data to all the Projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI057234-09
Application #
8377863
Study Section
Special Emphasis Panel (ZAI1-KS-I)
Project Start
Project End
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
9
Fiscal Year
2012
Total Cost
$162,095
Indirect Cost
$58,188
Name
Baylor Research Institute
Department
Type
DUNS #
145745022
City
Dallas
State
TX
Country
United States
Zip Code
75204
Todorova, Biliana; Salabert, Nina; Tricot, Sabine et al. (2017) Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques. Contrast Media Mol Imaging 2017:3127908
Athale, Shruti; Banchereau, Romain; Thompson-Snipes, LuAnn et al. (2017) Influenza vaccines differentially regulate the interferon response in human dendritic cell subsets. Sci Transl Med 9:
Mathew, Anuja (2017) Humanized mouse models to study human cell-mediated and humoral responses to dengue virus. Curr Opin Virol 25:76-80
Silvin, Aymeric; Yu, Chun I; Lahaye, Xavier et al. (2017) Constitutive resistance to viral infection in human CD141+ dendritic cells. Sci Immunol 2:
Yoshimatsu, Gumpei; Kunnathodi, Faisal; Saravanan, Prathab Balaji et al. (2017) Pancreatic ?-Cell-Derived IP-10/CXCL10 Isletokine Mediates Early Loss of Graft Function in Islet Cell Transplantation. Diabetes 66:2857-2867
Raymond, Donald D; Stewart, Shaun M; Lee, Jiwon et al. (2016) Influenza immunization elicits antibodies specific for an egg-adapted vaccine strain. Nat Med 22:1465-1469
Yin, Wenjie; Gorvel, Laurent; Zurawski, Sandra et al. (2016) Functional Specialty of CD40 and Dendritic Cell Surface Lectins for Exogenous Antigen Presentation to CD8(+) and CD4(+) T Cells. EBioMedicine 5:46-58
Blohmke, Christoph J; Darton, Thomas C; Jones, Claire et al. (2016) Interferon-driven alterations of the host's amino acid metabolism in the pathogenesis of typhoid fever. J Exp Med 213:1061-77
Schmitt, Nathalie; Liu, Yang; Bentebibel, Salah-Eddine et al. (2016) Molecular Mechanisms Regulating T Helper 1 versus T Follicular Helper Cell Differentiation in Humans. Cell Rep 16:1082-1095
Kovats, S; Turner, S; Simmons, A et al. (2016) West Nile virus-infected human dendritic cells fail to fully activate invariant natural killer T cells. Clin Exp Immunol 186:214-226

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