Flow cytometry has become an essential tool for investigators requiring high throughput analysis of single cells. Assessment of the phenotype and function of human T cells during and after a viral infection is a critical element to all CTRHIB projects (Research Projects 1-4 and Technology Development Project 1). The CTRHIB Flow Cytometry Core will provide flow cytometry services to support human immunology research by CTRHIB investigators. The Flow Cytometry Core will provide CTRHIB investigators with access to a state-of-the-art FACS ARIA flow cytometer to permit analysis and sorting of cells. Currently we are able to use 11 different flurochromes on a single sample thus allowing assessment of multiple parameters on a single cell. The core will provide training for scientists and laboratory staff to operate the FACS ARIA for data collection and analysis. It will also provide guidance in design and analysis of flow cytometry experiments, including techniques for cell staining, cell sorting and selection of antibody panels.

Public Health Relevance

Polychromatic flow cytometry allows for detailed measurements even with small sample sizes to assess a myriad of T cell functions. All four research projects, the technology development project and the clinical core will heavily depend on the use of the Flow Cytometry Core for the assessment of T cell phenotype and function.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1-KS-I)
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University of Massachusetts Medical School Worcester
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Canetta, Sarah E; Bao, Yuanyuan; Co, Mary Dawn T et al. (2014) Serological documentation of maternal influenza exposure and bipolar disorder in adult offspring. Am J Psychiatry 171:557-63
Thompson, Mikayla R; Sharma, Shruti; Atianand, Maninjay et al. (2014) Interferon ?-inducible protein (IFI) 16 transcriptionally regulates type i interferons and other interferon-stimulated genes and controls the interferon response to both DNA and RNA viruses. J Biol Chem 289:23568-81
Mathew, Anuja; Townsley, Elizabeth; Ennis, Francis A (2014) Elucidating the role of T cells in protection against and pathogenesis of dengue virus infections. Future Microbiol 9:411-25
Yin, Liusong; Trenh, Peter; Guce, Abigail et al. (2014) Susceptibility to HLA-DM protein is determined by a dynamic conformation of major histocompatibility complex class II molecule bound with peptide. J Biol Chem 289:23449-64
Parra, Miguel; Herrera, Daniel; Calvo-Calle, J Mauricio et al. (2014) Circulating human rotavirus specific CD4 T cells identified with a class II tetramer express the intestinal homing receptors ?4?7 and CCR9. Virology 452-453:191-201
Yin, Liusong; Stern, Lawrence J (2014) A novel method to measure HLA-DM-susceptibility of peptides bound to MHC class II molecules based on peptide binding competition assay and differential IC(50) determination. J Immunol Methods 406:21-33
Schmidt, Madelyn R; McGinnes-Cullen, Lori W; Kenward, Sarah A et al. (2014) Modification of the respiratory syncytial virus f protein in virus-like particles impacts generation of B cell memory. J Virol 88:10165-76
Terajima, Masanori; Co, Mary Dawn T; Ennis, Francis A (2014) Age and different influenza viruses. Lancet Infect Dis 14:101
Outinen, T K; Mäkelä, S; Huttunen, R et al. (2014) Urine soluble urokinase-type plasminogen activator receptor levels correlate with proteinuria in Puumala hantavirus infection. J Intern Med 276:387-95
Co, Mary Dawn T; Terajima, Masanori; Thomas, Stephen J et al. (2014) Relationship of preexisting influenza hemagglutination inhibition, complement-dependent lytic, and antibody-dependent cellular cytotoxicity antibodies to the development of clinical illness in a prospective study of A(H1N1)pdm09 Influenza in children. Viral Immunol 27:375-82

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