The major objective of the Mouse Model Core is to provide a centralized service-oriented facility that offers the capability of producing and preserving transgenic and knockout mice. For the AADCRC program, the Core is specially charged with supporting the development of cell-specific and conditional expression strategies, particularly for targeting airway epithelial cells. The Mouse Core will also serve the projects of this program by supporting the infrastructure of a mouse model of respiratory virus infection and postviral asthma that will be extensively utilized by the projects. In that regard, the Core will oversee the use of standardized protocols of this model and ensure regulatory compliance with use of the protocol. Furthermore, in association with this model and other transgenic mouse models of experimental asthma, the Mouse Model Core will provide methods for measuring lung resistance and compliance for determinations of airway reactivity. Thus, the Mouse Core will be an important part of this AADCRC program. The major components of the Mouse Core, including the transgenic/knockout production unit and the respiratory infection unit are each provided with dedicated space and state-of-the-art equipment within our animal barrier facility. Moreover, the personnel that staff the Core have generally worked in it since its inception in 1995 and thereby have an extensive track record of productivity. Over this period, the Mouse Core has been responsible for generating over 120 transgenic and knockout mouse lines. The Mouse Core was an integral part of this AADCRC program during the previous funding cycle and was again highly productive in supporting each of the previous projects. For the next cycle, the Core is also central to the approach of each Project. In addition the Core has and will continue to provide a substrate for shared mice and phenotyping resources that serve to facilitate collaborative interactions among project investigators in the AADCRC program.

National Institute of Health (NIH)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1)
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Washington University
Saint Louis
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Demehri, S; Yockey, L J; Visness, C M et al. (2014) Circulating TSLP associates with decreased wheezing in non-atopic preschool children: data from the URECA birth cohort. Clin Exp Allergy 44:851-7
Gu, Xiaoling; Karp, Philip H; Brody, Steven L et al. (2014) Chemosensory functions for pulmonary neuroendocrine cells. Am J Respir Cell Mol Biol 50:637-46
Patel, Dhara A; Patel, Anand C; Nolan, William C et al. (2014) High-throughput screening normalized to biological response: application to antiviral drug discovery. J Biomol Screen 19:119-30
Xu, Wei; Edwards, Megan R; Borek, Dominika M et al. (2014) Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of phosphorylated STAT1. Cell Host Microbe 16:187-200
Patel, Dhara A; You, Yingjian; Huang, Guangming et al. (2014) Interferon response and respiratory virus control are preserved in bronchial epithelial cells in asthma. J Allergy Clin Immunol 134:1402-1412.e7
Pennati, Francesca; Quirk, James D; Yablonskiy, Dmitriy A et al. (2014) Assessment of regional lung function with multivolume (1)H MR imaging in health and obstructive lung disease: comparison with (3)He MR imaging. Radiology 273:580-90
Kober, Daniel L; Wanhainen, Kelsey M; Johnson, Britney M et al. (2014) Preparation, crystallization, and preliminary crystallographic analysis of wild-type and mutant human TREM-2 ectodomains linked to neurodegenerative and inflammatory diseases. Protein Expr Purif 96:32-8
Pan, Jie-Hong; Adair-Kirk, Tracy L; Patel, Anand C et al. (2014) Myb permits multilineage airway epithelial cell differentiation. Stem Cells 32:3245-56
Holtzman, Michael J; Byers, Derek E; Alexander-Brett, Jennifer et al. (2014) The role of airway epithelial cells and innate immune cells in chronic respiratory disease. Nat Rev Immunol 14:686-98
Philippot, Quentin; Deslée, Gaëtan; Adair-Kirk, Tracy L et al. (2014) Increased iron sequestration in alveolar macrophages in chronic obstructive pulmonary disease. PLoS One 9:e96285

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