Flow cytometry is a central tool in most immunological research. The studies in this Program are no exception. Flow cytometry technology has rapidly advanced, however, and the capacity to extract more information from fewer and fewer cells continues to improve. The goals of this Core are to expand flow cytometry capabilities of this Program by providing custom labeled antibodies and technical expertise to take advantage of up to 20-parameter (18-color) flow cytometry and to produce and provide MHC class l/peptide tetramers to the individual Projects to facilitate examination of antiviral CDS T cell responses. These activities will enhance the ability of the Program to meet the goals and milestones outlined in the individual Projects and greatly expand the technical and experimental capabilities of the investigators. Thus, the specific Aims for this core are: 1) To develop and validate fluorochrome conjugated antibody reagents for multiparameter flow cytometry and optimize multiparameter flow cytometry panels;and 2) To generate and validate MHC tetramer reagents for CDS T cell responses in mice. The ability to have antibodies on nontraditional fluorescence channels will greatly expand the capabilities for projects where it would be beneficial to examine more parameters simultaneously and in experiments where cell numbers (e.g. from nonlymphoid tissues) are limited. Thus, Core C will provide essential and widely used reagents and services to this Program.

Public Health Relevance

Cutting edge capabilities to assess antiviral immunity are a crucial component of any efforts to understand the basic mechanisms of viral control. This Core will provide the Program with reagents and expertise to ensure the investigators are at the forefront of immunological and flow cytometry analysis of antiviral immunity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI083022-04
Application #
8377014
Study Section
Special Emphasis Panel (ZAI1-BDP-I)
Project Start
Project End
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
4
Fiscal Year
2012
Total Cost
$104,720
Indirect Cost
$25,287
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Wang, Y; Jiang, B; Guo, Y et al. (2017) Cross-protective mucosal immunity mediated by memory Th17 cells against Streptococcus pneumoniae lung infection. Mucosal Immunol 10:250-259
Thome, Joseph J C; Bickham, Kara L; Ohmura, Yoshiaki et al. (2016) Early-life compartmentalization of human T cell differentiation and regulatory function in mucosal and lymphoid tissues. Nat Med 22:72-7
Yang, Cheng; Khanniche, Asma; DiSpirito, Joanna R et al. (2016) Transcriptome Signatures Reveal Rapid Induction of Immune-Responsive Genes in Human Memory CD8(+) T Cells. Sci Rep 6:27005
Crosby, Erika J; Clark, Megan; Novais, Fernanda O et al. (2015) Lymphocytic Choriomeningitis Virus Expands a Population of NKG2D+CD8+ T Cells That Exacerbates Disease in Mice Coinfected with Leishmania major. J Immunol 195:3301-10
Yu, Minjun; Owens, David M; Ghosh, Sankar et al. (2015) Conditional PDK1 Ablation Promotes Epidermal and T-Cell-Mediated Dysfunctions Leading to Inflammatory Skin Disease. J Invest Dermatol 135:2688-2696
Zens, Kyra D; Farber, Donna L (2015) Memory CD4 T cells in influenza. Curr Top Microbiol Immunol 386:399-421
Weissler, Katherine A; Garcia, Victoria; Kropf, Elizabeth et al. (2015) Distinct modes of antigen presentation promote the formation, differentiation, and activity of foxp3+ regulatory T cells in vivo. J Immunol 194:3784-97
Turner, Damian L; Gordon, Claire L; Farber, Donna L (2014) Tissue-resident T cells, in situ immunity and transplantation. Immunol Rev 258:150-66
Schadler, Keri L; Crosby, Erika J; Zhou, Alice Yao et al. (2014) Immunosurveillance by antiangiogenesis: tumor growth arrest by T cell-derived thrombospondin-1. Cancer Res 74:2171-81
Stelekati, Erietta; Shin, Haina; Doering, Travis A et al. (2014) Bystander chronic infection negatively impacts development of CD8(+) T cell memory. Immunity 40:801-13

Showing the most recent 10 out of 53 publications