The goal of Core 3 (Genotyping) is to provide the infrastructure and technical expertise required to carryout the genetic-based studies proposed in the ICEMR. Core 3 will take advantage ofthe instrument infrastructure and the seven years of experience and expertise established by the Johns Hopkins Malaria Research Institute. Core 3 will provide services for the ICEMR investigators to generate high-quality genotyping, genomics and expression data using microarray, PCR and sequencing platforms. The Core will provide genotyping analysis of mosquito samples using PCR and lllumina platforms. Parasite samples will be genotyped employing the Affymetrix GeneChip and real time PCR platforms. Samples from human participants will be analyzed for multiplicity of infection using a nested PCR-based approach. Core 3 will facilitate in the analysis, interpretation and the integration ofthe data into the ICEMR database.

Public Health Relevance

Core 3 will provide the instrumentation and technical expertise to carryout the genotyping and expression analyses required to define the contribution of parasite, vector and human genetics to the transmission dynamics in the southern African ICEMR study sites.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI089680-04
Application #
8503403
Study Section
Special Emphasis Panel (ZAI1-AWA-M)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
4
Fiscal Year
2013
Total Cost
$57,236
Indirect Cost
$28,931
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Sutcliffe, Catherine G; Searle, Kelly; Matakala, Hellen K et al. (2016) Measles and Rubella Seroprevalence Among HIV-Infected And Uninfected Zambian Youth. Pediatr Infect Dis J :
Samuel, Michael; Oliver, Shüné V; Coetzee, Maureen et al. (2016) The larvicidal effects of black pepper (Piper nigrum L.) and piperine against insecticide resistant and susceptible strains of Anopheles malaria vector mosquitoes. Parasit Vectors 9:238
Huang, Yuzheng; Li, Wei; Lu, Wuguang et al. (2016) Cloning and in vitro characterization of a Schistosoma japonicum aquaglyceroporin that functions in osmoregulation. Sci Rep 6:35030
Kanyangarara, Mufaro; Mamini, Edmore; Mharakurwa, Sungano et al. (2016) Reduction in Malaria Incidence following Indoor Residual Spraying with Actellic 300 CS in a Setting with Pyrethroid Resistance: Mutasa District, Zimbabwe. PLoS One 11:e0151971
Dahan-Moss, Yael Leah; Koekemoer, Lizette Leonie (2016) Analysis of esterase enzyme activity in adults of the major malaria vector Anopheles funestus. Parasit Vectors 9:110
Stevenson, Jennifer C; Simubali, Limonty; Mbambara, Saidon et al. (2016) Detection of Plasmodium falciparum Infection in Anopheles squamosus (Diptera: Culicidae) in an Area Targeted for Malaria Elimination, Southern Zambia. J Med Entomol 53:1482-1487
Pinchoff, Jessie; Chaponda, Mike; Shields, Timothy M et al. (2016) Individual and Household Level Risk Factors Associated with Malaria in Nchelenge District, a Region with Perennial Transmission: A Serial Cross-Sectional Study from 2012 to 2015. PLoS One 11:e0156717
Guo, Suqin; He, Lishan; Tisch, Daniel J et al. (2016) Pilot testing of dipsticks as point-of-care assays for rapid diagnosis of poor-quality artemisinin drugs in endemic settings. Trop Med Health 44:15
Shields, Timothy; Pinchoff, Jessie; Lubinda, Jailos et al. (2016) Spatial and temporal changes in household structure locations using high-resolution satellite imagery for population assessment: an analysis in southern Zambia, 2006-2011. Geospat Health 11:410
Das, Smita; Muleba, Mbanga; Stevenson, Jennifer C et al. (2016) Habitat Partitioning of Malaria Vectors in Nchelenge District, Zambia. Am J Trop Med Hyg 94:1234-44

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