The importance ofthe Anopheles vector in malaria transmission is evident in the continued efforts to limit, modify, or eliminate populations of anophelines. In our epidemiologically diverse study sites (Peru, Brazil) we will focus on the molecular ecology ofthe most anthropophilic Neotropical malaria vector, Anopheles darlingi, and, identify additional local/regional vectors that our preliminary data suggest are important. We hypothesize that in the mining sites aroimd Puerto Maldonado in SE Peru, and in the new agricultural settlement, Remansinho, Acrelandia, as deforestation increases, so will the importance of An. darlingi in transmission and maintenance. This has not previously been carefully monitored, except along the Iquitos-Nauta HW, and we anticipate providing up-to-date risk assessment of each ofthe localities where we will work. The identification of adult female mosquitoes using standard keys is problematic, and PCR-ITS2-RFLP assays have been implemented on a local scale aroimd Iquitos. We will expand these assays to include our study sites in SE Peru and Acrelandia. Accurate species identification will provide information on changes in species composition over time, which also might alter risk of transmission. Using microsatellite markers that we have in hand (for An. darlingi) or will develop (for other local/regional vectors) we will test for changes in genotype, population structure and evidence of expansion, particularly in the most modified sites (mining, agriculture). An. darlingi is diverging into northem and southem lineages that we detected with the nuclear white gene. We developed a diagnostic PCR-RFLP assay to survey An. darlngi to determine the lineage distributions, because genetic differentiation could signify differences in vector competence, ecology or behavior. As bednets or ITNs become more acceptable, in some situations nets alone may provoke a behavioral change in the biting times of vector species, and the ITNs can influence levels of mosquito insecticide resistance. We will monitor peak biting times, biting rate, insecticide resistance, and changes in genetic diversity and effective population size to provide baseline data for evaluation, data that are currently unavailable in most ofthe Amazon. We will use a newly developed mosquito trap that reduces risk to collectors.

Public Health Relevance

Through this research, we expect to provide basic data to: accurately incriminate the primary malaria vector species in three regions, discover the impact of ecological changes (deforestafion and mining) on their population structure and behavior, and the evaluate the usefulness of ITNs or insecticide treatment to control An. dariingi and other vectors.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1)
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University of California San Diego
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Ferreira, Marcelo U; Castro, Marcia C (2016) Challenges for malaria elimination in Brazil. Malar J 15:284
Conn, Jan E (2016) News from Africa: Novel Anopheline Species Transmit Plasmodium in Western Kenya. Am J Trop Med Hyg 94:251-2
Nicolete, Vanessa C; Frischmann, Sarah; Barbosa, Susana et al. (2016) Naturally Acquired Binding-Inhibitory Antibodies to Plasmodium vivax Duffy Binding Protein and Clinical Immunity to Malaria in Rural Amazonians. J Infect Dis 214:1539-1546
Shaw-Saliba, Kathryn; Clarke, David; Santos, Jorge M et al. (2016) Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates. Int J Parasitol 46:679-83
Rosas-Aguirre, Angel; Gamboa, Dionicia; Manrique, Paulo et al. (2016) Epidemiology of Plasmodium vivax Malaria in Peru. Am J Trop Med Hyg :
Gonçalves-Lopes, Raquel M; Lima, Nathália F; Carvalho, Karina I et al. (2016) Surface expression of inhibitory (CTLA-4) and stimulatory (OX40) receptors by CD4(+) regulatory T cell subsets circulating in human malaria. Microbes Infect 18:639-648
Llanos-Chea, Fiorella; Martínez, Dalila; Rosas, Angel et al. (2015) Characteristics of Travel-Related Severe Plasmodium vivax and Plasmodium falciparum Malaria in Individuals Hospitalized at a Tertiary Referral Center in Lima, Peru. Am J Trop Med Hyg 93:1249-53
Bickersmith, Sara A; Lainhart, William; Moreno, Marta et al. (2015) A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines. Mem Inst Oswaldo Cruz 110:573-6
Kobayashi, Tamaki; Gamboa, Dionicia; Ndiaye, Daouda et al. (2015) Malaria Diagnosis Across the International Centers of Excellence for Malaria Research: Platforms, Performance, and Standardization. Am J Trop Med Hyg 93:99-109
Cui, Liwang; Mharakurwa, Sungano; Ndiaye, Daouda et al. (2015) Antimalarial Drug Resistance: Literature Review and Activities and Findings of the ICEMR Network. Am J Trop Med Hyg 93:57-68

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