This U19 project brings together a large number of investigators of varied expertise generating different forms of data to characterize immune responses to vaccinated and infected subjects in several sample collections. The resulting multidimensional dataset will be an extremely rich substrate for systematic analyses that not only answer specific questions but also investigate the possibility of coordinated responses to vaccination across multiple platforms. Leveraging existing database infrastructure at the Broad Institute, we will build a database for this U19 project by adding functionality that accommodates the unique features of different immunophenotypic data. In particular, we will implement an automated pipeline for cytometric data preparation, quality control, and analysis based on our FLAME program. This novel method models multidimensional data and extracts parameters such as geometric descriptions of cell population clusters and the orientation of these clusters in multidimensional space. This method illustrates the cutting-edge computational methods that we will deploy in this project. Additional methods will be written and implemented as needed in close collaboration with the Analysis Team, whose members are also physically located at the Broad Institute.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI089992-04
Application #
8667882
Study Section
Special Emphasis Panel (ZAI1-QV-I (M2))
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
4
Fiscal Year
2013
Total Cost
$95,664
Indirect Cost
$28,773
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Yao, Yi; Montgomery, Ruth R (2016) Role of Immune Aging in Susceptibility to West Nile Virus. Methods Mol Biol 1435:235-47
Montgomery, Ruth R (2016) High standards for high dimensional investigations. Cytometry A 89:886-888
Das, Rituparna; Loughran, Kerry; Murchison, Charles et al. (2016) Association between high expression macrophage migration inhibitory factor (MIF) alleles and West Nile virus encephalitis. Cytokine 78:51-4
Montgomery, Ruth R; Murray, Kristy O (2015) Risk factors for West Nile virus infection and disease in populations and individuals. Expert Rev Anti Infect Ther 13:317-25
Strauss-Albee, Dara M; Fukuyama, Julia; Liang, Emily C et al. (2015) Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility. Sci Transl Med 7:297ra115
Mohanty, Subhasis; Joshi, Samit R; Ueda, Ikuyo et al. (2015) Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults. J Infect Dis 211:1174-84
Qian, Feng; Goel, Gautam; Meng, Hailong et al. (2015) Systems immunology reveals markers of susceptibility to West Nile virus infection. Clin Vaccine Immunol 22:6-16
Qian, Feng; Montgomery, Ruth R (2015) Imaging Immunosenescence. Methods Mol Biol 1343:97-106
Thakar, Juilee; Mohanty, Subhasis; West, A Phillip et al. (2015) Aging-dependent alterations in gene expression and a mitochondrial signature of responsiveness to human influenza vaccination. Aging (Albany NY) 7:38-52
Tsioris, Konstantinos; Gupta, Namita T; Ogunniyi, Adebola O et al. (2015) Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing. Integr Biol (Camb) 7:1587-97

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