The Histology and Comparative Pathology (Core C) will provide the four projects of this U19 CMCR grant a comprehensive histopathology facility that will assist the investigators in the histological analysis required for the investigators to analyze the role of stem cells in improving the outcome of radiation induce gastrointestinal syndrome (RIGS), Radiation induced hematopoietic syndrome (RIHS), and Radiation-induced cutaneous syndrome (RICS). The core will provide advice on preparation and processing of samples, as well as on the techniques and procedures available for the different studies. The Core will provide necropsy and histopathology services for phenotypic analysis along with a full range of histological services The services offered by the core will include: protocol design, necropsy, including tissue fixation, tissue proceeding to paraffin block, frozen embedding, standard and specialized stains and immunohistochemieal analyses. It will also include histopathological evaluation of tissue samples utilizing a standardized method of analysis. The key personnel of the core will be the director and the histology technical staff. The director will be responsible for supervising all the activities of the core, offering advice on the different methods and procedures, interpretation of the results, and generating histopathology reports and interpretations of the data. The technical staff will prepare and process tissue samples as appropriate for the study at hand, perform some of the specialized techniques and procedures (e.g. immunohistochemistry and special stains). Significance: Appropriate tissue sample preparation (fixation, processing, trimming and staining) is essential to all projects in this Program Project as it allow the study of the in vivo impact of stem cell technologies on the outcome of radiation-induced injury.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI091175-04
Application #
8517568
Study Section
Special Emphasis Panel (ZAI1-KS-I)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
4
Fiscal Year
2013
Total Cost
$145,756
Indirect Cost
$57,951
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Alexeev, Vitali; Lash, Elizabeth; Aguillard, April et al. (2014) Radiation protection of the gastrointestinal tract and growth inhibition of prostate cancer xenografts by a single compound. Mol Cancer Ther 13:2968-77
Almo, Steven C; Guha, Chandan (2014) Considerations for combined immune checkpoint modulation and radiation treatment. Radiat Res 182:230-8
Agoni, Lorenzo; Basu, Indranil; Gupta, Seema et al. (2014) Rigosertib is a more effective radiosensitizer than cisplatin in concurrent chemoradiation treatment of cervical carcinoma, in vitro and in vivo. Int J Radiat Oncol Biol Phys 88:1180-7
Vainshtein, Jeffrey M; Kabarriti, Rafi; Mehta, Keyur J et al. (2014) Bone marrow-derived stromal cell therapy in cirrhosis: clinical evidence, cellular mechanisms, and implications for the treatment of hepatocellular carcinoma. Int J Radiat Oncol Biol Phys 89:786-803
Zachman, Derek K; Leon, Ronald P; Das, Prerna et al. (2013) Endothelial cells mitigate DNA damage and promote the regeneration of hematopoietic stem cells after radiation injury. Stem Cell Res 11:1013-21
Saha, Subhrajit; Bhanja, Payel; Liu, Laibin et al. (2012) TLR9 agonist protects mice from radiation-induced gastrointestinal syndrome. PLoS One 7:e29357
Vaitheesvaran, Bhavapriya; Yang, Li; Hartil, Kirsten et al. (2012) Peripheral effects of FAAH deficiency on fuel and energy homeostasis: role of dysregulated lysine acetylation. PLoS One 7:e33717
Yang, Li; Vaitheesvaran, Bhavapriya; Hartil, Kirsten et al. (2011) The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching. J Proteome Res 10:4134-49
Lv, Haitao; Palacios, Gustavo; Hartil, Kirsten et al. (2011) Advantages of tandem LC-MS for the rapid assessment of tissue-specific metabolic complexity using a pentafluorophenylpropyl stationary phase. J Proteome Res 10:2104-12
Saha, Subhrajit; Bhanja, Payel; Kabarriti, Rafi et al. (2011) Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice. PLoS One 6:e24072