The Lung Biospecimens Core will provide our AADCRC Program with processing of all blood and lung specimens to be collected for each project. This includes all tissue processing, isolation of cells from blood and bronchoalveolar lavage fluid. Isolation of DNA, RNA and miRNA, and performance of bioplex cytokine and chemokine assays. The Core is led by Anne Sperling, PhD, Associate Professor of Medicine, who has extensive experience in the administration of university-wide core facilities and deep familiarity with all the assays and isolations to be performed by this Core. A senior research technologist performs all assays and isolations and maintains rigorous quality control and record-keeping. Timely isolation and storage of biological materials is of the utmost importance in the successful completion of each of the projects in this program. This dedicated Core will meet these obligations.

Agency
National Institute of Health (NIH)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI095230-04
Application #
8691380
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
City
Chicago
State
IL
Country
United States
Zip Code
60637
Denner, Darcy R; Sangwan, Naseer; Becker, Julia B et al. (2016) Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways. J Allergy Clin Immunol 137:1398-1405.e3
Press, Valerie G; Arora, Vineet M; Trela, Kristin C et al. (2016) Effectiveness of Interventions to Teach Metered-Dose and Diskus Inhaler Techniques. A Randomized Trial. Ann Am Thorac Soc 13:816-24
Kessler, Michael D; Yerges-Armstrong, Laura; Taub, Margaret A et al. (2016) Challenges and disparities in the application of personalized genomic medicine to populations with African ancestry. Nat Commun 7:12521
Nicodemus-Johnson, Jessie; Myers, Rachel A; Sakabe, Noburu J et al. (2016) DNA methylation in lung cells is associated with asthma endotypes and genetic risk. JCI Insight 1:e90151
Mathias, Rasika Ann; Taub, Margaret A; Gignoux, Christopher R et al. (2016) A continuum of admixture in the Western Hemisphere revealed by the African Diaspora genome. Nat Commun 7:12522
Bønnelykke, Klaus; Ober, Carole (2016) Leveraging gene-environment interactions and endotypes for asthma gene discovery. J Allergy Clin Immunol 137:667-79
Ober, Carole (2016) Asthma Genetics in the Post-GWAS Era. Ann Am Thorac Soc 13 Suppl 1:S85-90
Stein, Michelle M; Hrusch, Cara L; Gozdz, Justyna et al. (2016) Innate Immunity and Asthma Risk in Amish and Hutterite Farm Children. N Engl J Med 375:411-21
Stevens, Whitney W; Grammer 3rd, Leslie C (2015) Occupational rhinitis: an update. Curr Allergy Asthma Rep 15:487
Kim, Kyung Won; Myers, Rachel A; Lee, Ji Hyun et al. (2015) Genome-wide association study of recalcitrant atopic dermatitis in Korean children. J Allergy Clin Immunol 136:678-684.e4

Showing the most recent 10 out of 32 publications