The primary objectives of the Biochemical Function Technology Core are to 1) produce and purify high-quality gene product targets, 2) perform biochemical/biophysical characterization of these targets, 3) conduct or facilitate the analysis of complex protein sample mixtures by mass spectrometry. Our technology core, located at Argonne National Lab, will support the gene function characterization effort of the investigators of our team by generating experimental evidence from biochemical assays that will help to determine the biochemical function(s) of gene products.

Public Health Relevance

Defining gene function requires experimentation across multiple scales. Our technology core is a state-of-the-art facility with the necessary advanced analytical instrumentation to define the biochemical function of purified gene products.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI107792-01
Application #
8597758
Study Section
Special Emphasis Panel (ZAI1-FDS-M (M1))
Project Start
Project End
Budget Start
2013-08-13
Budget End
2014-07-31
Support Year
1
Fiscal Year
2013
Total Cost
$145,545
Indirect Cost
$40,545
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Czy?, Daniel M; Willett, Jonathan W; Crosson, Sean (2017) Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen. J Bacteriol 199:
Herrou, Julien; Willett, Jonathan W; Czy?, Daniel M et al. (2017) Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria. J Bacteriol 199:
Baric, Ralph S; Crosson, Sean; Damania, Blossom et al. (2016) Next-Generation High-Throughput Functional Annotation of Microbial Genomes. MBio 7:
Herrou, Julien; Czy?, Daniel M; Willett, Jonathan W et al. (2016) WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System. J Bacteriol 198:1281-93
Willett, Jonathan W; Herrou, Julien; Czyz, Daniel M et al. (2016) Brucella abortus ?rpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis. Vaccine 34:5073-5081
Czy?, Daniel M; Jain-Gupta, Neeta; Shuman, Howard A et al. (2016) A dual-targeting approach to inhibit Brucella abortus replication in human cells. Sci Rep 6:35835
Meyer, Peter A; Socias, Stephanie; Key, Jason et al. (2016) Data publication with the structural biology data grid supports live analysis. Nat Commun 7:10882
Willett, Jonathan W; Herrou, Julien; Briegel, Ariane et al. (2015) Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen. Proc Natl Acad Sci U S A 112:E3709-18
Kim, Hye-Sook; Willett, Jonathan W; Jain-Gupta, Neeta et al. (2014) The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signalling pathway. Mol Microbiol 94:913-25
Tam, Christina; Demke, Owen; Hermanas, Timothy et al. (2014) YfbA, a Yersinia pestis regulator required for colonization and biofilm formation in the gut of cat fleas. J Bacteriol 196:1165-73

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