The primary objectives of the Biochemical Function Technology Core are to 1) produce and purify high-quality gene product targets, 2) perform biochemical/biophysical characterization of these targets, 3) conduct or facilitate the analysis of complex protein sample mixtures by mass spectrometry. Our technology core, located at Argonne National Lab, will support the gene function characterization effort of the investigators of our team by generating experimental evidence from biochemical assays that will help to determine the biochemical function(s) of gene products.
Defining gene function requires experimentation across multiple scales. Our technology core is a state-of-the-art facility with the necessary advanced analytical instrumentation to define the biochemical function of purified gene products.
|Tam, Christina; Demke, Owen; Hermanas, Timothy et al. (2014) YfbA, a Yersinia pestis regulator required for colonization and biofilm formation in the gut of cat fleas. J Bacteriol 196:1165-73|
|Kim, Hye-Sook; Willett, Jonathan W; Jain-Gupta, Neeta et al. (2014) The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signalling pathway. Mol Microbiol 94:913-25|