The primary role of the Administrative Core is to coordinate research activities among the four individual research projects, the Technology Core, and the Data Management and Resource Dissemination Core. High-quality functional annotation requires experimental effort on multiple scales, from the biochemical/biophysical to the cellular levels. We will combine experimental microbiological studies, in which we examine gene function in the pathogen, with in vitro biochemical analyses on purified gene products. To maximize our collective output the proper leadership and administrative structure must be in place. We recognize a need to set in place an effective environment to foster the full engagement of each investigator and their staffs, and to provide the scope of resources that not only facilitates, but also enhances individual research. The goal of the proposed administrative structure is """"""""efficient integration"""""""". This integration will bekey to our major goal of providing the research community with the highest quality functional assignment of as many uncharacterized genes as possible.
Our multi-scale approach to the determination of gene function is beyond the scope of an individual laboratory and must therefore occur within the auspices of a larger program. Appropriate administration of the research program will ensure that we attain our goal of the highest quality functional annotation of uncharacterized genes in NIAID category A and B priority pathogens.
|CzyÅ¼, Daniel M; Jain-Gupta, Neeta; Shuman, Howard A et al. (2016) A dual-targeting approach to inhibit Brucella abortus replication in human cells. Sci Rep 6:35835|
|Meyer, Peter A; Socias, Stephanie; Key, Jason et al. (2016) Data publication with the structural biology data grid supports live analysis. Nat Commun 7:10882|
|Herrou, Julien; CzyÅ¼, Daniel M; Willett, Jonathan W et al. (2016) WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System. J Bacteriol 198:1281-93|
|Baric, Ralph S; Crosson, Sean; Damania, Blossom et al. (2016) Next-Generation High-Throughput Functional Annotation of Microbial Genomes. MBio 7:|
|Willett, Jonathan W; Herrou, Julien; CzyÅ¼, Daniel M et al. (2016) Brucella abortus Î”rpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis. Vaccine 34:5073-81|
|Willett, Jonathan W; Herrou, Julien; Briegel, Ariane et al. (2015) Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen. Proc Natl Acad Sci U S A 112:E3709-18|
|Kim, Hye-Sook; Willett, Jonathan W; Jain-Gupta, Neeta et al. (2014) The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signalling pathway. Mol Microbiol 94:913-25|
|Tam, Christina; Demke, Owen; Hermanas, Timothy et al. (2014) YfbA, a Yersinia pestis regulator required for colonization and biofilm formation in the gut of cat fleas. J Bacteriol 196:1165-73|
|Herrou, Julien; Crosson, Sean (2013) Molecular structure of the Brucella abortus metalloprotein RicA, a Rab2-binding virulence effector. Biochemistry 52:9020-8|