The primary objectives of the Biochemical Function Technology Core are to 1) produce and purify high-quality gene product targets, 2) perform biochemical/biophysical characterization of these targets, 3) conduct or facilitate the analysis of complex protein sample mixtures by mass spectrometry. Our technology core, located at Argonne National Lab, will support the gene function characterization effort of the investigators of our team by generating experimental evidence from biochemical assays that will help to determine the biochemical function(s) of gene products.

Public Health Relevance

Defining gene function requires experimentation across multiple scales. Our technology core is a state-of-the-art facility with the necessary advanced analytical instrumentation to define the biochemical function of purified gene products.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1)
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University of Chicago
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Herrou, Julien; Willett, Jonathan W; Fiebig, Aretha et al. (2018) Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus. Mol Microbiol :
Tien, Matthew; Fiebig, Aretha; Crosson, Sean (2018) Gene network analysis identifies a central post-transcriptional regulator of cellular stress survival. Elife 7:
Herrou, Julien; Czy?, Daniel M; Fiebig, Aretha et al. (2018) Molecular control of gene expression by Brucella BaaR, an IclR-type transcriptional repressor. J Biol Chem 293:7437-7456
Czy?, Daniel M; Willett, Jonathan W; Crosson, Sean (2017) Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen. J Bacteriol 199:
Mitchell, Anthony; Tam, Christina; Elli, Derek et al. (2017) Glutathionylation of Yersinia pestis LcrV and Its Effects on Plague Pathogenesis. MBio 8:
Herrou, Julien; Willett, Jonathan W; Czy?, Daniel M et al. (2017) Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria. J Bacteriol 199:
Baric, Ralph S; Crosson, Sean; Damania, Blossom et al. (2016) Next-Generation High-Throughput Functional Annotation of Microbial Genomes. MBio 7:
Czy?, Daniel M; Jain-Gupta, Neeta; Shuman, Howard A et al. (2016) A dual-targeting approach to inhibit Brucella abortus replication in human cells. Sci Rep 6:35835
Willett, Jonathan W; Herrou, Julien; Czyz, Daniel M et al. (2016) Brucella abortus ?rpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis. Vaccine 34:5073-5081
Herrou, Julien; Czy?, Daniel M; Willett, Jonathan W et al. (2016) WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System. J Bacteriol 198:1281-93

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