The primary objectives of the Biochemical Function Technology Core are to 1) produce and purify high-quality gene product targets, 2) perform biochemical/biophysical characterization of these targets, 3) conduct or facilitate the analysis of complex protein sample mixtures by mass spectrometry. Our technology core, located at Argonne National Lab, will support the gene function characterization effort of the investigators of our team by generating experimental evidence from biochemical assays that will help to determine the biochemical function(s) of gene products.
Defining gene function requires experimentation across multiple scales. Our technology core is a state-of-the-art facility with the necessary advanced analytical instrumentation to define the biochemical function of purified gene products.
|CzyÅ¼, Daniel M; Jain-Gupta, Neeta; Shuman, Howard A et al. (2016) A dual-targeting approach to inhibit Brucella abortus replication in human cells. Sci Rep 6:35835|
|Meyer, Peter A; Socias, Stephanie; Key, Jason et al. (2016) Data publication with the structural biology data grid supports live analysis. Nat Commun 7:10882|
|Herrou, Julien; CzyÅ¼, Daniel M; Willett, Jonathan W et al. (2016) WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System. J Bacteriol 198:1281-93|
|Baric, Ralph S; Crosson, Sean; Damania, Blossom et al. (2016) Next-Generation High-Throughput Functional Annotation of Microbial Genomes. MBio 7:|
|Willett, Jonathan W; Herrou, Julien; CzyÅ¼, Daniel M et al. (2016) Brucella abortus Î”rpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis. Vaccine 34:5073-81|
|Willett, Jonathan W; Herrou, Julien; Briegel, Ariane et al. (2015) Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen. Proc Natl Acad Sci U S A 112:E3709-18|
|Kim, Hye-Sook; Willett, Jonathan W; Jain-Gupta, Neeta et al. (2014) The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signalling pathway. Mol Microbiol 94:913-25|
|Tam, Christina; Demke, Owen; Hermanas, Timothy et al. (2014) YfbA, a Yersinia pestis regulator required for colonization and biofilm formation in the gut of cat fleas. J Bacteriol 196:1165-73|
|Herrou, Julien; Crosson, Sean (2013) Molecular structure of the Brucella abortus metalloprotein RicA, a Rab2-binding virulence effector. Biochemistry 52:9020-8|