The overall objective of this core is to provide large-scale immunology and influenza Virology support to enhance the product development activities of the center investigators. The core will provide multiplex cytokine/chemokine profiling, high-throughput humoral response monitoring via antigen-specific ELISA and Surface Plasmon Resonance, and provide comprehensive influenza virology support (viral stocks, titers and neutralization assays). To accomplish this goal the expertise and efforts of Dr. Sempowski's cellular immunology lab and Dr. Ramsburg's viral-immunology lab have been combined. The inclusion on an Immunology/Influenza Virology Core will-provide a common set of platforms for mechanistic analysis of host immune response for all three center investigators. The use of ELISA/SPR will allow for monitoring host response to both experimental vaccines/adjuvants and challenge pathogens. Blood, tissue and culture supernatant inflammatory biomarker analysis will provide further valuable insight into specific host responses which will only enhance the challenge and mechanistic studies proposed by center projects to develop nanopartical vaccines and therapeutics to Flu and Dengue. Lastly, the inclusion of a centralized lab for maintenance of influenza virus strains and tittered stocks for all projects will allow for uniform flu studies across all projects. Moreover, the influenza support lab will work closely with investigators and collaborators to ensure standardized execution of influenza viral titers and neutralizing antibody responses to influenza.
This core will provide comprehensive immunology and influenza virology support to centralize and enhance the research and development activities of the center investigators. Specifically projects 1-3 will develop and test an innovative nanoparticle platform technology (PRINT) for antiviral vaccines and therapeutics. This highly focused program will investigate application of this technology to inhibiting Influenza and Dengue virus infection.
|Kai, Marc P; Brighton, Hailey E; Fromen, Catherine A et al. (2016) Tumor Presence Induces Global Immune Changes and Enhances Nanoparticle Clearance. ACS Nano 10:861-70|
|Fromen, Catherine A; Rahhal, Tojan B; Robbins, Gregory R et al. (2016) Nanoparticle surface charge impacts distribution, uptake and lymph node trafficking by pulmonary antigen-presenting cells. Nanomedicine 12:677-87|
|Fromen, Catherine A; Robbins, Gregory R; Shen, Tammy W et al. (2015) Controlled analysis of nanoparticle charge on mucosal and systemic antibody responses following pulmonary immunization. Proc Natl Acad Sci U S A 112:488-93|
|Cheong, Woo-Chang; Kang, Hye-Ri; Yoon, Hyunyee et al. (2015) Influenza A Virus NS1 Protein Inhibits the NLRP3 Inflammasome. PLoS One 10:e0126456|
|Roberts, Reid A; Eitas, Timothy K; Byrne, James D et al. (2015) Towards programming immune tolerance through geometric manipulation of phosphatidylserine. Biomaterials 72:1-10|
|Callaway, Justin B; Smith, Scott A; Widman, Douglas G et al. (2015) Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1Î² Secretion: A Primary Human Monocyte Model. PLoS One 10:e0136708|
|Callaway, Justin B; Smith, Scott A; McKinnon, Karen P et al. (2015) Spleen Tyrosine Kinase (Syk) Mediates IL-1Î² Induction by Primary Human Monocytes during Antibody-enhanced Dengue Virus Infection. J Biol Chem 290:17306-20|
|Robbins, Gregory R; Roberts, Reid A; Guo, Haitao et al. (2015) Analysis of human innate immune responses to PRINT fabricated nanoparticles with cross validation using a humanized mouse model. Nanomedicine 11:589-99|
|Mueller, Sarah N; Tian, Shaomin; DeSimone, Joseph M (2015) Rapid and Persistent Delivery of Antigen by Lymph Node Targeting PRINT Nanoparticle Vaccine Carrier To Promote Humoral Immunity. Mol Pharm 12:1356-65|