The selection of model ENMs used for the RESAC Center projects is largely driven by the over-riding hypothesis of the RESAC toxicological projects (Projects 1 and 2), that (i) the cellular, molecular and immune reactivities in response to ENMs depend on the specific physicochemical properties of the ENMs;(ii) the physicochemical properties of the ENMs deposited in the lungs critically depend on their interaction with lung lining fluid, which in turn impacts on ENM reactivity with macrophages and epithelial cells;(iii) in turn, this determines their entry, localization, biological activity and fate in the lung. The selection is also based on potentials for exposure and health risks. We plan to study two types of commercially widely-used ENMs: silver and carbon nanomaterials. Their specific physicochemical properties are summarized in Table SCI. Our selection of these two ENMs allows us to compare biological effects in two systems of great technological importance. By preparing well-controlled ENMs, standardized by particle size and aspect ratio, we will be able to move beyond the particular behaviour of a single chemically homogenous system (whether silver or carbon), in order to isolate, more fundamentally, the effects of geometry and chemistry.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19ES019536-04
Application #
8468716
Study Section
Special Emphasis Panel (ZES1-SET-V)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
4
Fiscal Year
2013
Total Cost
$98,007
Indirect Cost
Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Seiffert, Joanna; Buckley, Alison; Leo, Bey et al. (2016) Pulmonary effects of inhalation of spark-generated silver nanoparticles in Brown-Norway and Sprague-Dawley rats. Respir Res 17:85
Botelho, Danielle J; Leo, Bey Fen; Massa, Christopher B et al. (2016) Low-dose AgNPs reduce lung mechanical function and innate immune defense in the absence of cellular toxicity. Nanotoxicology 10:118-27
Govada, Lata; Leese, Hannah S; Saridakis, Emmanuel et al. (2016) Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals. Sci Rep 6:20053
Chen, S; Goode, A E; Skepper, J N et al. (2016) Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments. J Microsc 261:157-66
Marchetti, Magda; Shaffer, Milo S P; Zambianchi, Martina et al. (2015) Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size. Philos Trans R Soc Lond B Biol Sci 370:20140038
Seiffert, Joanna; Hussain, Farhana; Wiegman, Coen et al. (2015) Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726
Wang, Chun-Hua; Punde, Tushar Harishchandra; Huang, Chien-Da et al. (2015) Fibrocyte trafficking in patients with chronic obstructive asthma and during an acute asthma exacerbation. J Allergy Clin Immunol 135:1154-62.e1-5
Sweeney, Sinbad; Theodorou, Ioannis G; Zambianchi, Marta et al. (2015) Silver nanowire interactions with primary human alveolar type-II epithelial cell secretions: contrasting bioreactivity with human alveolar type-I and type-II epithelial cells. Nanoscale 7:10398-409
Sarkar, Srijata; Leo, Bey Fen; Carranza, Claudia et al. (2015) Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification. PLoS One 10:e0143077
Theodorou, Ioannis G; Botelho, Danielle; Schwander, Stephan et al. (2015) Static and Dynamic Microscopy of the Chemical Stability and Aggregation State of Silver Nanowires in Components of Murine Pulmonary Surfactant. Environ Sci Technol 49:8048-56

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