This proposal is a renewal of our EDRN Biomarker Reference Laboratory (BRL) which is located within the Clinical Chemistry Division (CCD) of the Johns Hopkins Medical Laboratories (JHML) at the Johns Hopkins Medical Institutions. The BRL will continue to serve as a network resource for clinical and laboratory validation of biomarkers, which includes technological developments and assay refinement. The JHML are certified by the College of American Pathologist (CAP) and regulated by CLIA (Clinical Laboratory Improvement Amendments of 1988). As such, the JHML follow stringent, good laboratory practice (GLP) guidelines for quality control and quality assurance. The proposed developmental project will be conducted at the Center for Biomaricer Discovery (CBD), The Johns Hopkins University. The project goal is to validate glycoproteins as biomarkers for the early detection of aggressive prostate cancer using cutting edge technologies such as mass spectrometry, protein/lectin microarrays, bioinformatics, and immunoassays. We have assembled a strong team of research and clinical scientists based on many years of experience with cancer biomarkers and in technology development, study design, bioinformatics, validation, and translation. Dr. Chan, the PI, is the Director of both CCD and CBD. He has over 30 years of experience in clinical chemistry and has conducted many (>50) research studies funded by industry on cancer diagnostics leading to the approval by the FDA for clinical use. Most recently, the first in vitro diagnostic multivariate index assay (IVDMIA) was cleared by the FDA for clinical use. This test, OVAl, was based on our original multiplex proteomic study published in Cancer Research. In addition, several leading scientists from the diagnostics industry will serve as consultants to provide expertise and facilitate the development and validation of cancer diagnostics. In this renewal, we plan to continue our strong commitment to the EDRN mission through network collaborations, and provide leadership in biomarker validation and technology/assay improvement. With this multi-disciplinary team of scientists, the BRL at Johns Hopkins offers the best opportunity for the success of cancer biomarker validation and translation into the clinic for the early detection of cancer.
As part of the EDRN network, the purpose of this Biomarker Reference Laboratory is to assist in the development and validation of laboratory assays in order to bring new biomarkers for the eariy detection of cancer from the laboratory setting to the ciinicai setting. We also propose to validate prostate cancer markers to aid in Identifying men who will most benefit from prostate cancer treatment.
|Hoofnagle, Andrew N; Whiteaker, Jeffrey R; Carr, Steven A et al. (2016) Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays. Clin Chem 62:48-69|
|Sun, Shisheng; Shah, Punit; Eshghi, Shadi Toghi et al. (2016) Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotechnol 34:84-8|
|Toghi Eshghi, Shadi; Yang, Weiming; Hu, Yingwei et al. (2016) Classification of Tandem Mass Spectra for Identification of N- and O-linked Glycopeptides. Sci Rep 6:37189|
|Yang, Weiming; Jackson, Brooks; Zhang, Hui (2016) Identification of glycoproteins associated with HIV latently infected cells using quantitative glycoproteomics. Proteomics 16:1872-80|
|de la Calle, Claire; Patil, Dattatraya; Wei, John T et al. (2015) Multicenter Evaluation of the Prostate Health Index to Detect Aggressive Prostate Cancer in Biopsy NaÃ¯ve Men. J Urol 194:65-72|
|Li, Qing Kay; Chen, Li; Ao, Ming-Hui et al. (2015) Serum fucosylated prostate-specific antigen (PSA) improves the differentiation of aggressive from non-aggressive prostate cancers. Theranostics 5:267-76|
|Shah, Punit; Zhang, Bai; Choi, Caitlin et al. (2015) Tissue proteomics using chemical immobilization and mass spectrometry. Anal Biochem 469:27-33|
|Li, Yan; Shah, Punit; De Marzo, Angelo M et al. (2015) Identification of glycoproteins containing specific glycans using a lectin-chemical method. Anal Chem 87:4683-7|
|Thomas, Stefani N; Harlan, Robert; Chen, Jing et al. (2015) Multiplexed Targeted Mass Spectrometry-Based Assays for the Quantification of N-Linked Glycosite-Containing Peptides in Serum. Anal Chem 87:10830-8|
|Loeb, Stacy; Sanda, Martin G; Broyles, Dennis L et al. (2015) The prostate health index selectively identifies clinically significant prostate cancer. J Urol 193:1163-9|
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