This proposal, from a group of established scientists at the University of Cincinnati, is in response to RFA DK-10-006 to continue funding for one of six NIH-funded Mouse Metabolic Phenotyping Centers with the mission of providing phenotyping services related to diabetes and its complications to diverse scientists and investigators. Five Cores are proposed: 1) Administrative/Statistical Core will be responsible for processing requests, uploading test results to the national database, perform statistical analyses, take care of budgetary issues, purchase of supplies and equipment, post information about the types of tests offered and protocol used on the Center's web site;2) Animal Care Core will receive, house, feed, and genotype the incoming mice as well as maintain them in excellent condition during the period of phenotypic testing;3) Lipid, Lipoprotein, and Glucose Metabolism Core will study lipid and glucose metabolism and measure gastrointestinal hormones known to influence insulin secretion;4) Cardiovascular Core will provide numerous assessments of cardiovascular complications and tissue inflammation;and 5) Energy Metabolism Core will assess simple and detailed parameters of energy intake (food intake) and energy expenditure (including use of indirect calorimetry) and determine body composition and fat content. We believe that our collective experience and expertise as well as our experience running and operating one of the six centers since July 1, 2001 makes our proposal unique and competitive. We are excited at the prospect of collaborating on this important initiative.

Public Health Relevance

Diabetes is a major health problem, and investigation of many aspects of its physiology and pathophysiology are ongoing. Numerous transgenic and knockout mouse models are being generated for the investigation of both Types I &II diabetes and their co-morbidities. MMPCs will provide detailed metabolic phenotyping of these mouse models and will be potentially useful for understanding diabetes and its complications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
2U24DK059630-11
Application #
8192192
Study Section
Special Emphasis Panel (ZDK1-GRB-S (M1))
Program Officer
Abraham, Kristin M
Project Start
2001-03-01
Project End
2016-06-30
Budget Start
2011-09-16
Budget End
2012-06-30
Support Year
11
Fiscal Year
2011
Total Cost
$531,202
Indirect Cost
Name
University of Cincinnati
Department
Pathology
Type
Schools of Medicine
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Packard, Amy E B; Zhang, Jintao; Myers, Brent et al. (2017) Apolipoprotein A-IV constrains HPA and behavioral stress responsivity in a strain-dependent manner. Psychoneuroendocrinology 86:34-44
Hinder, Lucy M; O'Brien, Phillipe D; Hayes, John M et al. (2017) Dietary reversal of neuropathy in a murine model of prediabetes and metabolic syndrome. Dis Model Mech 10:717-725
Li, Xiaoming; Wang, Fei; Xu, Min et al. (2017) ApoA-IV improves insulin sensitivity and glucose uptake in mouse adipocytes via PI3K-Akt Signaling. Sci Rep 7:41289
Zhang, Yupeng; He, Jing; Zhao, Jing et al. (2017) Effect of ApoA4 on SERPINA3 mediated by nuclear receptors NR4A1 and NR1D1 in hepatocytes. Biochem Biophys Res Commun 487:327-332
Costa, Diana K; Huckestein, Brydie R; Edmunds, Lia R et al. (2016) Reduced intestinal lipid absorption and body weight-independent improvements in insulin sensitivity in high-fat diet-fed Park2 knockout mice. Am J Physiol Endocrinol Metab 311:E105-16
Rider, T; LeBoeuf, R C; Tso, Patrick et al. (2016) The Use of Kits in the Analysis of Tissue Lipids Requires Validation. Lipids 51:497-504
Kassis, Timothy; Yarlagadda, Sri Charan; Kohan, Alison B et al. (2016) Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity. Am J Physiol Gastrointest Liver Physiol 310:G776-89
Sato, Hirokazu; Zhang, Linda S; Martinez, Kristina et al. (2016) Antibiotics Suppress Activation of Intestinal Mucosal Mast Cells and Reduce Dietary Lipid Absorption in Sprague-Dawley Rats. Gastroenterology 151:923-932
Yan, Chunling; He, Yanlin; Xu, Yuanzhong et al. (2016) Apolipoprotein A-IV Inhibits AgRP/NPY Neurons and Activates Pro-Opiomelanocortin Neurons in the Arcuate Nucleus. Neuroendocrinology 103:476-488
McNamara, Robert K; Jandacek, Ronald; Tso, Patrick et al. (2016) Adolescents with or at ultra-high risk for bipolar disorder exhibit erythrocyte docosahexaenoic acid and eicosapentaenoic acid deficits: a candidate prodromal risk biomarker. Early Interv Psychiatry 10:203-11

Showing the most recent 10 out of 165 publications