The UMass Mouse Metabolic Phenotyping Center involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic characterization of transgenic mice using unique and standardized techniques mostly involving, in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of cardiovascular complications using non-invasive procedures in mice. The UMass MMPC consists of 3 Phenotyping Cores: 1) Metabolism Core applies hyperinsullnemic-euglycemic clamp to assess insulin sensitivity and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic beta-cell function in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, and 1H-MRS to measure fat/lean/water mass in mice, 2) Analytical Core uses Luminex. Meso Scale Discovery, and Cobas Clinical Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, performs molecular experiments to examine insulin signaling, and conducts histological studies to examine tissues, 3) Cardiovascular Complications Core uses Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging, and CODA blood pressure device to examine cardiovascular abnormalities associated with diabetes. Mice provided by the users are overseen by the Animal Care Core that processes the receiving, quarantine, and housing of mice. Lastly, financial and administrative operation, research &development program, and website &database are managed by the Administrative Core. Overall, the aim of the UMass MMPC is to become an NIH-funded national center that offers elegant and standardized techniques and scientific expertise using a fee-for-service structure to characterize the metabolic and organ function phenotypes of transgenic mice and provide important insights into the mechanism of diabetes and its complications.

Public Health Relevance

Prevalence of diabetes is increasing at an alarming rate, and by year 2025. One out of 3 Americans is expected to be diabetic. Using established expertise, elegant experimental procedures, and state-of-the-art instruments, our goal is to understand how diabetes develops and to identify a cure to treat obesity and diabetes.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Resource-Related Research Projects--Cooperative Agreements (U24)
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Special Emphasis Panel (ZDK1-GRB-S (M1))
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Abraham, Kristin M
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University of Massachusetts Medical School Worcester
Other Basic Sciences
Schools of Medicine
United States
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Vernia, Santiago; Cavanagh-Kyros, Julie; Garcia-Haro, Luisa et al. (2014) The PPAR?-FGF21 hormone axis contributes to metabolic regulation by the hepatic JNK signaling pathway. Cell Metab 20:512-25
Tao, Hanlin; Zhang, Yong; Zeng, Xiangang et al. (2014) Niclosamide ethanolamine-induced mild mitochondrial uncoupling improves diabetic symptoms in mice. Nat Med 20:1263-9
Mori, Marcelo A; Thomou, Thomas; Boucher, Jeremie et al. (2014) Altered miRNA processing disrupts brown/white adipocyte determination and associates with lipodystrophy. J Clin Invest 124:3339-51
Pan, Dongning; Mao, Chunxiao; Quattrochi, Brian et al. (2014) MicroRNA-378 controls classical brown fat expansion to counteract obesity. Nat Commun 5:4725
Jung, Dae Young; Ko, Hwi Jin; Lichtman, Eben I et al. (2013) Short-term weight loss attenuates local tissue inflammation and improves insulin sensitivity without affecting adipose inflammation in obese mice. Am J Physiol Endocrinol Metab 304:E964-76
Hong, Eun-Gyoung; Kim, Brian W; Jung, Dae Young et al. (2013) Cardiac expression of human type 2 iodothyronine deiodinase increases glucose metabolism and protects against doxorubicin-induced cardiac dysfunction in male mice. Endocrinology 154:3937-46
Han, Myoung Sook; Jung, Dae Young; Morel, Caroline et al. (2013) JNK expression by macrophages promotes obesity-induced insulin resistance and inflammation. Science 339:218-22
Guo, Chang-An; Kogan, Sophia; Amano, Shinya U et al. (2013) CD40 deficiency in mice exacerbates obesity-induced adipose tissue inflammation, hepatic steatosis, and insulin resistance. Am J Physiol Endocrinol Metab 304:E951-63
Meng, Zhuo-Xian; Li, Siming; Wang, Lin et al. (2013) Baf60c drives glycolytic metabolism in the muscle and improves systemic glucose homeostasis through Deptor-mediated Akt activation. Nat Med 19:640-5
Jung, Dae Young; Chalasani, Umadevi; Pan, Ning et al. (2013) KLF15 is a molecular link between endoplasmic reticulum stress and insulin resistance. PLoS One 8:e77851

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