The development of effective strategies to prevent and treat HIV infection is of paramount importance to improve global human health. Simian immunodeficiency virus (SIV) infection of rhesus macaques (Macaca mulatta) is the most effective pre-clinical model of HIV infection. Members of the Macaca genus, however, harbor several viruses that could confound experimental results from studies relating to the AIDS prevention or cure. It is therefore vital to ensure a constant supply of specific pathogen free (SPF) rhesus macaques for experimental studies in AIDS research. Since 2002, the SPF Rhesus macaque breeding groups at Yerkes National Primate Research Center (YNPRC) have, in part, been supported by U24 funding. By the end of 2013, all breeding groups will be free of SIV, Simian T-Lymphotropic Virus (STLV), Simian Type D Retroviruses (SRV), and Herpes Simian B Virus (Herpes-B). The central aim of this final phase of U24 funding is to achieve financial sustainability for those U24-supported groups and to expand multi-generational, genetically characterized breeding groups to provide appropriately aged experimental animals for NIH funded AIDS research. To achieve this aim, proven management practices will be employed that allow optimum production of animals in socially stable breeding groups to provide animals to AIDS-related studies.
A second aim of this project is to expand methodology for SRV and Herpes testing in the Yerkes Virology Core. On-site SRV and Herpes B testing will save costs and allow a more rapid colony management response should the virus status of any SPF animal be questionable.
A third aim of this renewal is to transition to a more cost-effective, therefore financially sustainable, SNP-based and direct sequencing approach for macaque genotyping, providing AIDS investigators with pedigreed animals with confirmed sets of major histocompatibility complex (MHC). It is expected that the YNPRC SPF rhesus macaque breeding colony will be financially sustainable by the end of the next U24 funding period and will be recognized as a national resource of genetically well-characterized rhesus macaques available for HIV/AIDS research.

Public Health Relevance

The growth of the fully pedigreed, SPF AIDS-designated colony at the YNPRC will provide a supply of high-quality and well-characterized rhesus macaques for HIV/AIDS investigators at Emory University and elsewhere to help identify treatments to reduce the health burden imposed by AIDS in people.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
2U24OD011023-11
Application #
8542039
Study Section
Special Emphasis Panel (ZTR1-CM-6 (01))
Program Officer
Contreras, Miguel A
Project Start
2002-09-30
Project End
2017-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
11
Fiscal Year
2013
Total Cost
$1,475,188
Indirect Cost
$645,388
Name
Emory University
Department
Neurosciences
Type
Other Domestic Higher Education
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Lu, Wuxun; Wan, Yanmin; Ma, Fangrui et al. (2017) Distinct transcriptome profiles of Gag-specific CD8+ T cells temporally correlated with the protection elicited by SIV?nef live attenuated vaccine. PLoS One 12:e0173929
Pauthner, Matthias; Havenar-Daughton, Colin; Sok, Devin et al. (2017) Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches. Immunity 46:1073-1088.e6
McGary, C S; Alvarez, X; Harrington, S et al. (2017) The loss of CCR6+ and CD161+ CD4+ T-cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal Immunol 10:1082-1096
Rahmberg, Andrew R; Rajakumar, Premeela A; Billingsley, James M et al. (2017) Dynamic Modulation of Expression of Lentiviral Restriction Factors in Primary CD4+ T Cells following Simian Immunodeficiency Virus Infection. J Virol 91:
Kasturi, Sudhir Pai; Kozlowski, Pamela A; Nakaya, Helder I et al. (2017) Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5? Restrictive Macaques. J Virol 91:
Mylvaganam, Geetha H; Rios, Daniel; Abdelaal, Hadia M et al. (2017) Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A 114:1976-1981
Xue, Cheng; Raveendran, Muthuswamy; Harris, R Alan et al. (2016) The population genomics of rhesus macaques (Macaca mulatta) based on whole-genome sequences. Genome Res 26:1651-1662
Yee, JoAnn L; Vanderford, Thomas H; Didier, Elizabeth S et al. (2016) Specific pathogen free macaque colonies: a review of principles and recent advances for viral testing and colony management. J Med Primatol 45:55-78
Kanthaswamy, Sree; Johnson, Zachary; Trask, Jessica Satkoski et al. (2014) Development and validation of a SNP-based assay for inferring the genetic ancestry of rhesus macaques (Macaca mulatta). Am J Primatol 76:1105-13
Zimin, Aleksey V; Cornish, Adam S; Maudhoo, Mnirnal D et al. (2014) A new rhesus macaque assembly and annotation for next-generation sequencing analyses. Biol Direct 9:20

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