The objective of this proposal is to maintain a closed specific pathogen-free (SPF) rhesus macaque breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically-characterized Indian-origin monkeys will be donated by CPRC and consists of approximately 300 SPF rhesus macaques. Animals from this closed colony will be housed in reproduction corncribs, and will be quarterly tested for the presence of four target viruses (Herpes B, STLV-1, SIV and SRV). To characterize the colony and to optimize the structure of its breeding groups, a panel of highly polymorphic genetic markers will be used maximizing the level of their genetic heterogeneity. It is projected the program will produce a total offspring of 584 SPF rhesus macaques to help meet the increased demand for these animals. At the end of this grant, it is expected that this program will produce and will make available to PHS-funded investigators about 180 MHC typed SPF animals/year, thus attaining self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research projects.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Resource-Related Research Projects--Cooperative Agreements (U24)
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National Center for Research Resources Initial Review Group (RIRG)
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Contreras, Miguel A
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University of Puerto Rico Med Sciences
Other Domestic Higher Education
San Juan
United States
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Sariol, Carlos A; Martínez, Melween I; Rivera, Francheska et al. (2011) Decreased dengue replication and an increased anti-viral humoral response with the use of combined Toll-like receptor 3 and 7/8 agonists in macaques. PLoS One 6:e19323
Sariol, Carlos A; Galib, Yussef; Pantoja, Petraleigh et al. (2009) Fatal granulomatous meningoencephalitis associated to mycobacterium mucogenicum-like microorganism: a case report. P R Health Sci J 28:276-80
Sariol, Carlos A; Munoz-Jordan, Jorge L; Abel, Kristina et al. (2007) Transcriptional activation of interferon-stimulated genes but not of cytokine genes after primary infection of rhesus macaques with dengue virus type 1. Clin Vaccine Immunol 14:756-66
Sariol, C A; Gonzalez-Martinez, J; Arana, T et al. (2006) Differential distribution of antibodies to different viruses in young animals in the free-ranging rhesus macaques of Cayo Santiago. J Med Primatol 35:369-75
Sariol, Carlos A; Arana, Teresa; Maldonado, Elizabeth et al. (2005) Herpes B-virus seroreactivity in a colony of Macaca mulatta: data from the Sabana Seca Field Station, a new specific pathogen-free program. J Med Primatol 34:13-9