Due to the completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain long term. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of three MMRRC centers that has been developing this resource for the benefit of the entire biomedical community. Since initial funding began in 1999 and was renewed in 2005, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted the first strain and as of 2009,1639 strains have been archived by the three centers and are available for distribution. In parallel with these efforts, the MMRRC-UNC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, mutant gnotobiotic strains, and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) Streamline procedures to import, archive and distribute genetically modified mouse strains, (2) Investigate the mechanisms of oxidative damage in cryopreserved mouse sperm, and (3) archive and integrate Collaborative Cross mice as an MMRRC collection.

Public Health Relevance

(provided by applicant): In order to improve our understanding of human disease susceptibility and the implications this has for medicine, research must focus on gene function by inducing mouse mutations and then studying the resulting cellular or physiological abnormalities. The MMRRC imports, archives and distributes these important mutant strains to the scientific community for addressing the genetics underlying human disease conditions.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-CM-9 (01))
Program Officer
Mirochnitchenko, Oleg
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of North Carolina Chapel Hill
Schools of Medicine
Chapel Hill
United States
Zip Code
Morgan, Andrew P; Fu, Chen-Ping; Kao, Chia-Yu et al. (2015) The Mouse Universal Genotyping Array: From Substrains to Subspecies. G3 (Bethesda) 6:263-79
Morgan, Andrew P; Welsh, Catherine E (2015) Informatics resources for the Collaborative Cross and related mouse populations. Mamm Genome 26:521-39
Fedoriw, Andrew M; Menon, Debashish; Kim, Yuna et al. (2015) Key mediators of somatic ATR signaling localize to unpaired chromosomes in spermatocytes. Development 142:2972-80
Morgan, Andrew P (2015) argyle: An R Package for Analysis of Illumina Genotyping Arrays. G3 (Bethesda) 6:281-6
Mu, Weipeng; Starmer, Joshua; Fedoriw, Andrew M et al. (2014) Repression of the soma-specific transcriptome by Polycomb-repressive complex 2 promotes male germ cell development. Genes Dev 28:2056-69
Rogala, Allison R; Morgan, Andrew P; Christensen, Alexis M et al. (2014) The Collaborative Cross as a resource for modeling human disease: CC011/Unc, a new mouse model for spontaneous colitis. Mamm Genome 25:95-108
Gray, Jeffrey E; Starmer, Joshua; Lin, Vivian S et al. (2013) Mitochondrial hydrogen peroxide and defective cholesterol efflux prevent in vitro fertilization by cryopreserved inbred mouse sperm. Biol Reprod 89:17
Kim, Yuna; Fedoriw, Andrew M; Magnuson, Terry (2012) An essential role for a mammalian SWI/SNF chromatin-remodeling complex during male meiosis. Development 139:1133-40
Collaborative Cross Consortium (2012) The genome architecture of the Collaborative Cross mouse genetic reference population. Genetics 190:389-401
Donahue, Leah Rae; Hrabe de Angelis, Martin; Hagn, Michael et al. (2012) Centralized mouse repositories. Mamm Genome 23:559-71