The objective of this proposal is to maintain a closed specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically-characterized Indian-origin monkeys donated by CPRC will consist of approximately 755 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques of which 164 (or 22%) will also be free of the papova virus SV40. Thus, this colony will consist of two SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these two closed SPF sub colonies will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. A panel of highly polymorphic genetic markers will be used to characterize the colony and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. By year five, the program will produce a total estimated offspring of 1,082 SPF rhesus macaques, of which 301 will be also SV40 negative. These SPF macaques will help meet the increased research demand for these animals. At the end of this 5 year grant, it is expected that this program will produce more than 160 animals/year and will attain self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research initiatives.

Public Health Relevance

There is a high demand of SPF monkeys for biomedical research, and specifically for AIDS-related research programs. With the support of this grant, CPRC will continue to provide high quality Indian-origin SPF rhesus macaques to NIH/PHS-sponsored research programs and grants.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
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Special Emphasis Panel (ZRR1-CM-4 (01))
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Contreras, Miguel A
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University of Puerto Rico Med Sciences
Other Domestic Higher Education
San Juan
United States
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Kanthaswamy, Sreetharan; Oldt, Robert F; Ng, Jillian et al. (2018) Determination of major histocompatibility class I and class II genetic composition of the Caribbean Primate Center specific pathogen-free rhesus macaque (Macaca mulatta) colony based on massively parallel sequencing. J Med Primatol 47:379-387
Kanthaswamy, Sreetharan; Ng, Jillian; Hernández-Pacheco, Raisa et al. (2016) The Population Genetic Composition of Conventional and SPF Colonies of Rhesus Macaques (Macaca mulatta) at the Caribbean Primate Research Center. J Am Assoc Lab Anim Sci 55:147-51
Messer, William B; de Alwis, Ruklanthi; Yount, Boyd L et al. (2014) Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity. Proc Natl Acad Sci U S A 111:1939-44
Anglero-Rodriguez, Yesseinia I; Pantoja, Petraleigh; Sariol, Carlos A (2014) Dengue virus subverts the interferon induction pathway via NS2B/3 protease-I*B kinase epsilon interaction. Clin Vaccine Immunol 21:29-38
White, Laura J; Sariol, Carlos A; Mattocks, Melissa D et al. (2013) An alphavirus vector-based tetravalent dengue vaccine induces a rapid and protective immune response in macaques that differs qualitatively from immunity induced by live virus infection. J Virol 87:3409-24