The objective of this proposal is to maintain a closed specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically-characterized Indian-origin monkeys donated by CPRC will consist of approximately 755 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques of which 164 (or 22%) will also be free of the papova virus SV40. Thus, this colony will consist of two SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these two closed SPF sub colonies will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. A panel of highly polymorphic genetic markers will be used to characterize the colony and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. By year five, the program will produce a total estimated offspring of 1,082 SPF rhesus macaques, of which 301 will be also SV40 negative. These SPF macaques will help meet the increased research demand for these animals. At the end of this 5 year grant, it is expected that this program will produce more than 160 animals/year and will attain self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research initiatives.

Public Health Relevance

There is a high demand of SPF monkeys for biomedical research, and specifically for AIDS-related research programs. With the support of this grant, CPRC will continue to provide high quality Indian-origin SPF rhesus macaques to NIH/PHS-sponsored research programs and grants.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
5U42OD011128-13
Application #
8467775
Study Section
Special Emphasis Panel (ZRR1-CM-4 (01))
Program Officer
Contreras, Miguel A
Project Start
2000-09-30
Project End
2016-04-30
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
13
Fiscal Year
2013
Total Cost
$1,064,946
Indirect Cost
$350,020
Name
University of Puerto Rico Med Sciences
Department
None
Type
Other Domestic Higher Education
DUNS #
948108063
City
San Juan
State
PR
Country
United States
Zip Code
00936
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Messer, William B; de Alwis, Ruklanthi; Yount, Boyd L et al. (2014) Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity. Proc Natl Acad Sci U S A 111:1939-44
White, Laura J; Sariol, Carlos A; Mattocks, Melissa D et al. (2013) An alphavirus vector-based tetravalent dengue vaccine induces a rapid and protective immune response in macaques that differs qualitatively from immunity induced by live virus infection. J Virol 87:3409-24