Abstract

The objective of this proposal is to maintain a closed specific pathogen free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically characterized Indian-origin monkeys will be donated by CPRC and will consist of approximately 457 SPF rhesus macaques. Animals from this closed colony will be housed in reproduction corncribs, and will be quarterly tested for the presence of four target viruses (Herpes B, STLV-1, SIV and SRV). A panel of highly polymorphic genetic markers will be used to characterize the colony and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. By year five, the program will produce a total estimated offspring of 833 SPF rhesus macaques, which will help meet the increased demand for these animals. At the end of this grant, it is expected that this program will produce about 210 animals/year, thus attaining self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research initiatives.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
5U42RR016021-10
Application #
7797410
Study Section
Special Emphasis Panel (ZRR1-CM-7 (01))
Program Officer
Harding, John D
Project Start
2000-09-30
Project End
2011-03-31
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
10
Fiscal Year
2010
Total Cost
$926,827
Indirect Cost

  Institution

Name
University of Puerto Rico Med Sciences
Department
Type
Other Domestic Higher Education
DUNS #
948108063
City
San Juan
State
PR
Country
United States
Zip Code
00936

  Publications

Rodriguez-Mercado, Rafael; Ford, Gregory D; Xu, Zhenfeng et al. (2012) Acute neuronal injury and blood genomic profiles in a nonhuman primate model for ischemic stroke. Comp Med 62:427-38
Sariol, Carlos A; Martínez, Melween I; Rivera, Francheska et al. (2011) Decreased dengue replication and an increased anti-viral humoral response with the use of combined Toll-like receptor 3 and 7/8 agonists in macaques. PLoS One 6:e19323
Sariol, Carlos A; Galib, Yussef; Pantoja, Petraleigh et al. (2009) Fatal granulomatous meningoencephalitis associated to mycobacterium mucogenicum-like microorganism: a case report. P R Health Sci J 28:276-80
Mejía, A F; Gierbolini, L; Jacob, B et al. (2009) Pediatric hepatic hemangiosarcoma in a rhesus macaque (Macaca mulatta). J Med Primatol 38:121-4
Satkoski, J; George, D; Smith, D G et al. (2008) Genetic characterization of wild and captive rhesus macaques in China. J Med Primatol 37:67-80
Sariol, Carlos A; Munoz-Jordan, Jorge L; Abel, Kristina et al. (2007) Transcriptional activation of interferon-stimulated genes but not of cytokine genes after primary infection of rhesus macaques with dengue virus type 1. Clin Vaccine Immunol 14:756-66
Sariol, C A; Gonzalez-Martinez, J; Arana, T et al. (2006) Differential distribution of antibodies to different viruses in young animals in the free-ranging rhesus macaques of Cayo Santiago. J Med Primatol 35:369-75
Kanthaswamy, Sreetharan; von Dollen, Andrea; Kurushima, Jennifer D et al. (2006) Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta). Am J Primatol 68:73-95
Sariol, Carlos A; Arana, Teresa; Maldonado, Elizabeth et al. (2005) Herpes B-virus seroreactivity in a colony of Macaca mulatta: data from the Sabana Seca Field Station, a new specific pathogen-free program. J Med Primatol 34:13-9