Abstract

NERCE: Mass Spec Resource - Stephen Calderwood, M.D.The LTQ XL is a fast scanning tandem mass spectrometer, capable of sequencing several peptidesevery second, allowing rapid characterization and quantification (using isotope labels) of proteins frommicrobes. The instrument also has several intermediate scan modes, allowing for neutral loss analysis ofphosphate groups, as well as some medium full scan resolution modes for charge state assessment. Theinstrument has a higher duty cycle than the LTQ-FT, and a higher sensitivity for lower level peptides.Use of this instrument is prioritized for New England investigators working on NIAID Priority Pathogens,for which rapid identification of proteins and peptides will facilitate basic and applied science of relevant tothe missions of NERCE, such as the identification of pathogen proteins that are potential targets forantimicrobial development. This resource is publicized to NERCE investigators via e-mail, the NERCEWebsite, and direct communication when appropriate.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
3U54AI057159-05S1
Application #
7645376
Study Section
Special Emphasis Panel (ZAI1-NBS-M (M2))
Project Start
2008-03-01
Project End
2009-02-28
Budget Start
2008-03-01
Budget End
2009-02-28
Support Year
5
Fiscal Year
2008
Total Cost
$177,521
Indirect Cost

  Institution

Name
Harvard University
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

de Wispelaere, Melissanne; Lian, Wenlong; Potisopon, Supanee et al. (2018) Inhibition of Flaviviruses by Targeting a Conserved Pocket on the Viral Envelope Protein. Cell Chem Biol 25:1006-1016.e8
Huang, Nai-Jia; Pishesha, Novalia; Mukherjee, Jean et al. (2017) Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin. Nat Commun 8:423
Mertins, Philipp; Przybylski, Dariusz; Yosef, Nir et al. (2017) An Integrative Framework Reveals Signaling-to-Transcription Events in Toll-like Receptor Signaling. Cell Rep 19:2853-2866
Nair, Dhanalakshmi R; Chen, Ji; Monteiro, João M et al. (2017) A quinolinol-based small molecule with anti-MRSA activity that targets bacterial membrane and promotes fermentative metabolism. J Antibiot (Tokyo) 70:1009-1019
Choo, Min-Kyung; Sano, Yasuyo; Kim, Changhoon et al. (2017) TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects. J Exp Med 214:1297-1311
de Wispelaere, Mélissanne; Carocci, Margot; Liang, Yanke et al. (2017) Discovery of host-targeted covalent inhibitors of dengue virus. Antiviral Res 139:171-179
Umetsu, Dale T (2017) Mechanisms by which obesity impacts upon asthma. Thorax 72:174-177
Zheng, Huiqing; Colvin, Christopher J; Johnson, Benjamin K et al. (2017) Inhibitors of Mycobacterium tuberculosis DosRST signaling and persistence. Nat Chem Biol 13:218-225
Coulson, Garry B; Johnson, Benjamin K; Zheng, Huiqing et al. (2017) Targeting Mycobacterium tuberculosis Sensitivity to Thiol Stress at Acidic pH Kills the Bacterium and Potentiates Antibiotics. Cell Chem Biol 24:993-1004.e4
Arvanitis, Marios; Li, Gang; Li, De-Dong et al. (2016) A Conformationally Constrained Cyclic Acyldepsipeptide Is Highly Effective in Mice Infected with Methicillin-Susceptible and -Resistant Staphylococcus aureus. PLoS One 11:e0153912

Showing the most recent 10 out of 417 publications