NERCE Core B: Biomolecule Production, Robin Ross, Ph.D., Harvard Medical School.As part of the New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases(NERCE/BEID), the Biomolecule Production Core Laboratory provides services for production of medium tolarge scale amounts of purified bacterial, mammalian, and viral biomolecules to investigators conductingbiodefense research. This core facility is a state-of-the-art laboratory designed to produce both recombinantproteins and native carbohydrate molecules for use in structural analyses and vaccine formulation. Thisincludes, but is not restricted to, investigators in the following categories: those making use of theProteomics and Small Molecule Screening Core Laboratories who need to produce sufficient quantities ofidentified proteins to accomplish proposed screens; those developing vaccines containing bacterialcarbohydrate components who need large quantities of antigen for analysis in vitro biologic assays andanimal studies; and those already expressing target proteins in small scale using recombinant systems whoneed larger amounts of recombinant protein to expand their research. Investigators provide datademonstrating efficacy of production and purification methods as well as strains or recombinant plasmidpreparations where applicable. The laboratory scales-up these protocols for production and purification of100's of mg of the target biomolecule that is delivered to the investigator with a report outlining the methodsused. Since the Biomolecule Production Core opened in 2004, we have collaborated with 19 investigatorsfrom 8 research institutions and one company in the production of over 154 different biomolecules. We haveconducted 339 fermentation runs for the production of biomolecules, generating 52 Kg of bacterial cells.More than 277 purifications have been completed and we have delivered to investigators 34 Kg of cell paste,453 mg of purified carbohydrate, and 3.1 g of various purified recombinant protein.
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