The primary goal of Project 3 is to transfer and disseminate the CMPD expertise and technology to the broader scientific community and provide access to the processing capabilities, thereby increasing the scientific impact of PSI:Biology. A particular preference will be given towards human or eukaryotic membrane proteins given the expertise and platform developed by the CMPD. Accomplishing this goal will increase the rate of successful membrane protein structure determination and more importantly to increase the impact of structural genomics efforts in answering important biomedical questions. Our collaborative experiences on membrane proteins have shown us that the most effective way to accomplish key breakthroughs are to work with researchers who are dedicated and passionate about a specific subfamily of membrane proteins (e.g. ion channels, GPCRs, monotopic enzymes, transporters). We will operate Project 3 with the ?family specific dedication? philosophy in mind. In addition to solving structures, Project 3 will also implement a vigorous community outreach program that will share expertise and HT resources used in the structure determination of membrane proteins. Thus, Project 3 will undertake two primary tasks to achieve its goal;the first is processing of network targets nominated by groups under the Consortia for High-throughput Enabled Structural Biology Partnerships and those nominated by the general community. The second is an outreach program that we envision will be beneficial not just to the groups that we will be serving, but more importantly, to the CMPD as we view outreach as opportunity for strengthening our processes and protocols through feedback provided by external groups. With Project 3, we are leveraging the breakthroughs that were enabled through technology innovation and developments in PSI-1 and JCIMPT, and have been integrated with biological knowledge in PSI-2, to expand this approach beyond the initial GPCR targets listed in Project 1.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center--Cooperative Agreements (U54)
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Special Emphasis Panel (ZGM1-CBB-3)
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Scripps Research Institute
La Jolla
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Eddy, Matthew T; Didenko, Tatiana; Stevens, Raymond C et al. (2016) β2-Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop. Structure 24:2190-2197
Rowe, Timothy B; Luo, Zhe-Xi; Ketcham, Richard A et al. (2016) X-ray computed tomography datasets for forensic analysis of vertebrate fossils. Sci Data 3:160040
Ngo, Tony; Kufareva, Irina; Coleman, James L J et al. (2016) Identifying ligands at orphan GPCRs: current status using structure-based approaches. Br J Pharmacol :
Kufareva, Irina; Gustavsson, Martin; Holden, Lauren G et al. (2016) Disulfide Trapping for Modeling and Structure Determination of Receptor: Chemokine Complexes. Methods Enzymol 570:389-420
Yang, Dehua; de Graaf, Chris; Yang, Linlin et al. (2016) Structural Determinants of Binding the Seven-transmembrane Domain of the Glucagon-like Peptide-1 Receptor (GLP-1R). J Biol Chem 291:12991-3004
Zheng, Yi; Qin, Ling; Zacarías, Natalia V Ortiz et al. (2016) Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists. Nature 540:458-461
Zhu, Lan; Weierstall, Uwe; Cherezov, Vadim et al. (2016) Serial Femtosecond Crystallography of Membrane Proteins. Adv Exp Med Biol 922:151-60
White, Thomas A; Barty, Anton; Liu, Wei et al. (2016) Serial femtosecond crystallography datasets from G protein-coupled receptors. Sci Data 3:160057
Batyuk, Alexander; Galli, Lorenzo; Ishchenko, Andrii et al. (2016) Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Sci Adv 2:e1600292
Leach, Katie; Gregory, Karen J; Kufareva, Irina et al. (2016) Towards a structural understanding of allosteric drugs at the human calcium-sensing receptor. Cell Res 26:574-92

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